Psoriasis is a common chronic autoimmune skin disease involving the activation of T cells. To explore the proteomic signature of peripheral blood mononuclear cells, a quantitative analysis of their global proteome was conducted in samples from Chinese patients with new-onset psoriasis (n = 31) and healthy controls (n = 32) using an integrated quantitative approach with tandem mass tag labeling and LC-MS/MS. Protein annotation, unsupervised hierarchical clustering, functional classification, functional enrichment and cluster, and protein-protein interaction analyses were performed. A total of 5178 proteins were identified, of which 4404 proteins were quantified. The fold-change cutoff was set at 1.2 (patients vs controls); 335 proteins were upregulated, and 107 proteins were downregulated. The bioinformatics analysis indicated that the differentially expressed proteins were involved in processes related to the activation of immune cells including the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway, cellular energy metabolism, and proliferation. Three upregulated proteins and two phosphorylated proteins in the NF-κB pathway were verified or identified by Western blotting. These results confirm that the NF-κB pathway is critical to psoriasis. In addition, many differentially expressed proteins identified in this study have never before been associated with psoriasis, and further studies on these proteins are necessary.

No abstract

Objective: To observe the therapeutic effect of Leyin (~, LY) in treating psoriasis maculata (PM) and its effects on cellular immunity and vaginal epithelial cell mitosis in experimental mice. Methods: In this clinical study, 260 out-patients of PM were randomly divided into the observed group (n = 160) and the control group (n= 100), who were treated with oral intake of LY and Yinxieling granule (YXL, /~,l~,~ilJ) respectively to observe the therapeutic effect and the adverse reaction. And a followup study was also conducted on them. In the experimental study, delayed allergic model mice were treated with immunosuppressor(cyclophosphamide) and LY separately to observe the effects on their immune function and inhibition of vaginal epithelial cell proliferation. Results: In the observed group, 8 patients (5%) were cured, 32 (20~) basically cured, 48 (30~) markedly improved, 48 (30~) improved and 24 (15 ~) unchanged, the total effective rate being 85 ~, while in the control group, the corresponding numbers were 4, 8, 14, 38, 36 and 64~ respectively, with the comparison of the total effective rate between the two groups showing significant difference(P%0.01). Adverse reactions were not found in the study. Experimental study showed that LY could enhance the immune function of mice and had a certain inhibitory effect on vaginal epithelial cell proliferation. Conclusion: LY could treat PM effectively and shows immune enhancing and anti-mitosis effect in mice. KEY WORDS Leyin, macular psoriasis, cellular immunity, mice * This article is supported by the fund of Integrated Chinese Traditional and Western Medicine from Health Bureau of Tianjin City (No. 98012)

Tricholemmoma is a benign follicular tumor with differentiation towards cells of the outer hair root sheath and usually presents as a small papule on the face in middle-aged or older people. We herein report a case of solitary tricholemmoma located in the lateral right thigh, which shows the appearance of infiltrated plaque. Histopathologic examination is confirmed the diagnosis of tricholemmoma.