Modern, enantioselective catalyst development is driven largely by empiricism. Although this approach has fostered the introduction of most of the existing synthetic methods, it is inherently limited by the skill, creativity, and chemical intuition of the practitioner. Herein, we present a complementary approach to catalyst optimization in which statistical methods are used at each stage to streamline development. To construct the optimization informatics workflow, a number of critical components had to be subjected to rigorous validation. First, the critically important molecular descriptors were validated in two case studies to establish the importance of conformation-dependent molecular representations. Next, with a large data set available, it was possible to investigate the amount of data necessary to make predictive models with different modeling methods. Given the commercial availability of many catalyst structures, it was possible to compare models generated with algorithmically selected training sets and commercially available training sets. Finally, the augmentation of limited data sets is demonstrated in a method informed by unsupervised learning to restore the accuracy of the generated models.

Sterilization is the process of killing all microorganisms, while disinfection is the process of killing or removing all kinds of pathogenic microorganisms except bacterial spores. Biomaterials involved in cell experiments, animal experiments, and clinical applications need to be in the aseptic state, but their physical and chemical properties as well as biological activities can be affected by sterilization or disinfection. Decellularized matrix (dECM) is the low immunogenicity material obtained by removing cells from tissues, which retains many inherent components in tissues such as proteins and proteoglycans. But there are few studies concerning the effects of sterilization or disinfection on dECM, and the systematic introduction of sterilization or disinfection for dECM is even less. Therefore, this review systematically introduces and analyzes the mechanism, advantages, disadvantages, and applications of various sterilization and disinfection methods, discusses the factors influencing the selection of sterilization and disinfection methods, summarizes the sterilization and disinfection methods for various common dECM, and finally proposes a graphical route for selecting an appropriate sterilization or disinfection method for dECM and a technical route for validating the selected method, so as to provide the reference and basis for choosing more appropriate sterilization or disinfection methods of various dECM.

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Two Gram-negative, rod-shaped, gliding and pink-pigmented bacterial strains, X14-1 T and X19-1, were isolated from a mixture of sand samples collected from the desert of Xinjiang, China, and characterized by using a polyphasic taxonomic approach. Strains X14-1 T and X19-1 contained MK-7 as the predominant menaquinone. The major cellular fatty acids included iso-C 15 : 0 , iso-C 17 : 0 3-OH, summed feature 3 and summed feature 4.