n e Grid2003 Project has deployed a multi-virfual organization, application-driven grid laboratory ('"Grid3'7 that has sustained for several months the production-level services required by physics experiments of the Large Hadron Collider at CERN (ATLAS and CMS), the Sloan Digital Sky Survey project, the gravitational wave search experiment LIGO, the BTeV mperiment at Fermilab, as well as applications in molecular structure analysis and genome analysis, and computer science research projects in such areas as job and data scheduling. The deployed infiastmcture has been operating since Noisniber 2003 with 27 sites, apeak of 2800 processors, work loads fiom 10 different applications exceeding 1300 simultaneous jobs, and data transfers among sites of greater than 2 TBiday. We describe the principles that have guided the development of this unique infrastructure and the practical experiences that have resultedfiom its creation and use. We discuss application requirements for grid services deployment and con$guration. monitoring infiastnic fure, application performance, metrics. and operational experiences. We also summarize lessons learned.

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Background Since the coronavirus disease 2019 (COVID-19) outbreak, many COVID-19 variants have emerged, causing several waves of pandemics and many infections. Long COVID-19, or long-term sequelae after recovery from COVID-19, has aroused worldwide concern because it reduces patient quality of life after rehabilitation. We aimed to characterize the functional differential profile of the oral and gut microbiomes and serum metabolites in patients with gastrointestinal symptoms associated with long COVID-19. Methods We prospectively collected oral, fecal, and serum samples from 983 antibiotic-naïve patients with mild COVID-19 and performed a 3-month follow-up postdischarge. Forty-five fecal and saliva samples, and 25 paired serum samples were collected from patients with gastrointestinal symptoms of long COVID-19 at follow-up and from healthy controls, respectively. Eight fecal and saliva samples were collected without gastrointestinal symptoms of long COVID-19 at follow-up. Shotgun metagenomic sequencing of fecal samples and 2bRAD-M sequencing of saliva samples were performed on these paired samples. Two published COVID-19 gut microbiota cohorts were analyzed for comparison. Paired serum samples were analyzed using widely targeted metabolomics. Results Mild COVID-19 patients without gastrointestinal symptoms of long COVID-19 showed little difference in the gut and oral microbiota during hospitalization and at follow-up from healthy controls. The baseline and 3-month samples collected from patients with gastrointestinal symptoms associated with long COVID-19 showed significant differences, and ectopic colonization of the oral cavity by gut microbes including 27 common differentially abundant genera in the Proteobacteria phylum, was observed at the 3-month timepoint. Some of these bacteria, including Neisseria, Lautropia, and Agrobacterium, were highly related to differentially expressed serum metabolites with potential toxicity, such as 4-chlorophenylacetic acid, 5-sulfoxymethylfurfural, and estradiol valerate. Conclusions Our study characterized the changes in and correlations between the oral and gut microbiomes and serum metabolites in patients with gastrointestinal symptoms associated with long COVID-19. Additionally, our findings reveal that ectopically colonized bacteria from the gut to the oral cavity could exist in long COVID-19 patients with gastrointestinal symptoms, with a strong correlation to some potential harmful metabolites in serum.

Alongside the rise of ‘last-mile’ delivery in contemporary urban logistics, drones have demonstrate commercial potential, given their outstanding triple-bottom-line performance. However, as a lithium-ion battery-powered device, drones’ social and environmental merits can be overturned by battery recycling and disposal. To maintain economic performance, yet minimise environmental negatives, fleet sharing is widely applied in the transportation field, with the aim of creating synergies within industry and increasing overall fleet use. However, if a sharing platform’s transparency is doubted, the sharing ability of the platform will be discounted. Known for its transparent and secure merits, blockchain technology provides new opportunities to improve existing sharing solutions. In particular, the decentralised structure and data encryption algorithm offered by blockchain allow every participant equal access to shared resources without undermining security issues. Therefore, this study explores the implementation of a blockchain-enabled fleet sharing solution to optimise drone operations, with consideration of battery wear and disposal effects. Unlike classical vehicle routing with fleet sharing problems, this research is more challenging, with multiple objectives (i.e., shortest path and fewest charging times), and considers different levels of sharing abilities. In this study, we propose a mixed-integer programming model to formulate the intended problem and solve the problem with a tailored branch-and-price algorithm. Through extensive experiments, the computational performance of our proposed solution is first articulated, and then the effectiveness of using blockchain to improve overall optimisation is reflected, and a series of critical influential factors with managerial significance are demonstrated.