Slp forms a crystalline array of proteins on the outermost envelope of bacteria and archaea with a molecular weight of 40-200 kDa. Slp can self-assemble on the surface of liposomes in a proper environment via electrostatic interactions, which could be employed to functionalize liposomes by forming Slp-coated liposomes for various applications. Among the molecular characteristics, the stability, adhesion, and immobilization of biomacromolecules are regarded as the most meaningful. Compared to plain liposomes, Slp-coated liposomes show excellent physicochemical and biological stabilities. Recently, Slp-coated liposomes were shown to specifically adhere to the gastrointestinal tract, which was attributed to the "ligand-receptor interaction" effect. Furthermore, Slp as a "bridge" can immobilize functional biomacromolecules on the surface of liposomes via protein fusion technology or intermolecular forces, endowing liposomes with beneficial functions. In view of these favorable features, Slp-coated liposomes are highly likely to be an ideal platform for drug delivery and biomedical uses. This review aims to provide a general framework for the structure and characteristics of Slp and the interactions between Slp and liposomes, to highlight the unique properties and drug delivery as well as the biomedical applications of the Slp-coated liposomes, and to discuss the ongoing challenges and perspectives.

Background: This study sought to assess the application of three-dimensional (3D) printing in preoperative planning for anomalous pulmonary venous connection (APVC).Methods: From November 2017 to January 2019, 17 children diagnosed with APVC were enrolled in this study (total APVC supracardiac type in 10 children, intracardiac type in 2 children, infracardiac type in 1 child, mixed type in 1 child, partial APVC in 3 children). The age was ranged 2 days to 20 months old (median age 1 m 5 d). Before operation, 3D-printed patient-specific heart models were created based on a cardiac computed tomography (CCT) data set with photosensitive resin materials in stereolithography (SLA) technology. These 3D models were used for presurgical decision making and navigation in the operation room. After surgery, the roles of the 3D models were evaluated with questionnaires.Results: All 17 children successfully underwent surgeries. 3D heart models accurately demonstrated the malformations, which were all confirmed consistent with surgery findings. The final surgery programs were in accord with presurgical planning. Modeling took 0.5-2 h, with an average of 0.9±0.4 h. Printing took 2-5 h, with an average of 3.4±1.2 h. All these children were discharged without adverse events. During follow-up, 2 children suspect of anastomotic stenosis were performed cardiac CT, and 3D printed heart models were created. Results confirmed there was no obvious anastomotic stenosis. Questionnaire results indicate that 3D printing is a promising technique in clinical practice.Conclusions: 3D printing is beneficial for preoperative planning and post-surgery follow-up in APVC.

BackgroundTo investigate the accuracy and feasibility of CT in quantification of ventricular volume based on semiautomatic three-dimensional (3D) threshold-based segmentation in porcine heart and children with tetralogy of Fallot (TOF).MethodsEight porcine hearts were used in the study. The atria were resected and both ventricles of the eight porcine hearts were filled with solidifiable silica gel and performed CT scanning. The water displacement volume of silica gel casting mould was referred as gold standard of ventricular volume. Results of left and right ventricular volumes measured by CT were compared with reference standard. Twenty-three children diagnosed with TOF were retrospectively included. The ventricular volumetric parameters were assessed by cardiac CT before and 6 months after surgery.ResultsLeft ventricular and right ventricular volumes of porcine hearts measured by CT were highly correlated to casting mould (r=0.845, p=0.008; r=0.933, p=0.001), and there were no statistically significant differences (t=−1.059, p=0.325; t=−1.121, p=0.299). In children with TOF, right ventricular end-systole volumes 6 months after operation were higher than that before surgery, 21.93±4.44 vs 19.80±4.52 mL/m2, p=0.001. Right ventricular ejection fractions 6 months after surgery were lower compared with that before surgery 59.79%±4.26% vs 63.05%±5.04%, p=0.000.ConclusionsCT is able to accurately assess ventricular volumetric parameters based on semiautomatic 3D threshold-based segmentation. Both of the right and left ventricular volumetric parameters could be evaluated by CT in children with TOF.

BackgroundIntraoperative hypothermia (core temperature <36.0°C) is common during the perioperative period and can result in adverse consequences, especially in children. We aimed to determine the incidence of intraoperative hypothermia and its risk factors in pediatric patients during burn surgery.MethodsIn the present study we enrolled 197 pediatric patients with burn injury undergoing surgical debridement and skin grafting. Factors, such as total burn surface area (TBSA), were collected and analyzed to identify the potential risk factors for intraoperative hypothermia.ResultsThe incidence of intraoperative hypothermia among all patients was 17.8%. Compared with patients with normothermia, children with hypothermia were associated with larger TBSA (25% vs 15%, p<0.001) and with less intraoperative active warming (34.28% vs 54.93%, p<0.05). In addition, compared with patients with moderate-degree burn, patients with severe and extremely severe burn were associated with much higher risk of intraoperative hypothermia [severe: odds ratio (OR)=3.805, 95% confidence interval (CI)=1.396-10.368, p=0.009; extremely severe: OR=6.933, 95% CI=2.604-18.462, p<0.001]. TBSA was the only independent risk factor that emerged as being strongly associated with intraoperative hypothermia (OR=1.068, p=0.001) and could be used to predict the occurrence of hypothermia when combined with other factors. TBSA for predicting intraoperative hypothermia by receiver operating characteristic (ROC) curve analysis showed a good predictive ability with an area under the ROC curve of 0.758.ConclusionTBSA is an important risk factor for intraoperative hypothermia in pediatric patients with burn.