Objective. To study the effect of a multi-image source 3D modeling imaging examination system on the diagnosis of cardiovascular diseases in cardiac surgery. Methods. The data of 680 confirmed patients and 1590 suspected patients in the cardiac surgery department of all hospitals of a large chain hospital management group were selected. All patients gave the examination results of multiple image sources and independent examination results of multiple image sources, respectively, their examination sensitivity, specificity, and reliability were compared, and the treatment efficiency and nursing satisfaction of the virtual reference group were deduced in MATLAB. Perform the bivariate t -test and comparative statistics in SPSS. Results. The multi-image source 3D modeling examination system had higher examination sensitivity, specificity, and reliability and higher examination sensitivity in the early stage of the disease. It was deduced that the clinical efficiency and nursing satisfaction based on the examination results were significantly improved ( t < 10.000 , p < 0.01 ). Conclusion. The multi-image source 3D modeling imaging examination system is suitable for the diagnosis of cardiovascular diseases in cardiac surgery.