RESUMENCon el objetivo de evaluar la eficacia antihelmíntica de las presentaciones empleadas en los equinos de un rancho cubano, se realizó un test de reducción del recuento fecal de huevos de estróngilos (TRCFH) frente al albendazol y a la ivermectina, administrados vía oral. Se seleccionaron 33 equinos (albendazol 18; ivermectina 15). La reducción del conteo de huevos frente al albendazol varió entre 38% para el albendazol micronizado y 33% para el albendazol sulfóxido. La eficacia de la ivermectina fue del 100%. Posteriormente, a cuatro equinos con altos recuentos de huevos de los grupos tratados con albendazol se les hizo una segunda desparasitación con ivermectina en el día 17 posterior a la dosificación inicial para colectar en las heces los nematodos resistentes al benzimidazol. Estos nematodos fueron identificados morfológicamente. Del total de nematodos colectados, se identificaron 493 pertenecientes a las especies Cylicocyclus nassatus, Cyathostomum catinatum, Cylicostephanus longibursatus, Cylicostephanus goldi y Cylicostephanus minutus, las cuales representaron el 78.5, 7.7, 6.5, 3.9 y 3.4%, respectivamente. Este fue el primer reporte de C. minutus en equinos de Cuba. Se concluye que al menos cinco de las especies de ciatostomas fueron resistentes al albendazol.Palabras clave: albendazol; ciatostomas; eficacia; resistencia ABSTRACTThis study aimed to evaluate the efficacy of anthelmintic used in an equine ranch in Cuba. The faecal egg count reduction test of Strongyles (FECRT) was used to evaluate albendazole and ivermectin. Thirty-three horses were selected (18 treated with albendazole,

Cyathostomins are the most common and important group of large intestine nematodes, infecting horses worldwide. The current control strategy is associated with the development of anthelmintic resistance, which has been reported worldwide. Therefore, experiments with this family of parasites have become progressively important to provide their monitoring and control strategies. The aim of the present study was to propose a faster and more economic assay for isolation of genomic DNA from the adult stage of Cyathostomin parasites than reported. Adult parasites were collected from a single horse from a farm in São José dos Pinhais, PR, Brazil, and were identified. Genomic DNA was isolated from ten individual female adult parasites using a standardized procedure developed. Then, extraction from ten individual female was carried out by another DNA extraction method. DNA concentration from both methods were measured and compared. We obtained a good DNA quality with this standardized procedure. As a result of this analysis, we propose a modified phenol-chloroform method, which will contribute to assays that require DNA extraction from adult worms for genomic DNA sequences of cyathostomin, or species-specific identification.