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Novel two-dimensional Ti 3 C 2 MXene nanosheets were successfully prepared by etching Al from Ti 3 AlC 2 in LiF/HCl system. In order to further improve the dispersing property and electrical conductivity of Ti 3 C 2 nanosheets, Ti 3 C 2 /graphene oxide (Ti 3 C 2 -GO) nanocomposites were synthesized and applied for the fabrication of inkjet-printed hydrogen peroxide (H 2 O 2 ) sensor. The results of electrochemical characterization show that the prepared sensor maintains the biological activity of hemoglobin (Hb) and can be applied to the practical detection. The printed sensors display a dynamic range from 2 μM to 1 mM and a detection limit of 1.95 μM with a high sensitivity and excellent selectivity for H 2 O 2 determination. Therefore, the printable Ti 3 C 2 -GO nanocomposites are an excellent sensing platform for electrochemical determination.