Objectives-Delayed graft function (DGF) is a common early complication after kidney transplantation. The aim of the present study was to evaluate the value of contrast-enhanced ultrasonography (CEUS) in the early prediction of DGF after kidney transplantation.Methods-A total of 89 renal transplant recipients were retrospectively enrolled and divided into DGF group or normal graft function (NGF) group according to the allograft function. Conventional Doppler ultrasound and CEUS examination data on the first postoperative day were collected and analyzed.Results-The resistive indices of segmental and interlobar artery in the DGF group were significantly higher than those in the NGF group (0.71 AE 0.17 versus 0.63 AE 0.08, P = .006; 0.70 AE 0.16 versus 0.62 AE 0.08, P = .004, respectively). The patients experiencing DGF had significantly lower PI-c (14.7 dB AE 6.1 dB versus 18.5 dB AE 3.3 dB, P = .001) and smaller AUC-c (779.8 AE 375.8 dBÁseconds versus 991.0 AE 211.7 dBÁseconds, P = .003), as well as significantly lower PI-m (12.6 dB AE 5.9 dB versus 15.9 dB AE 3.9 dB, P = .006), shorter MTT-m (30.7 AE 9.4 seconds versus 36.3 AE 7.1 seconds, P = .01), and smaller AUC-m (P = .007). Multivariate analysis demonstrated that PI-c, AUC-c, and MTT-m were independent risk factors for DGF. The area under the receiver operating characteristic curve values of the combined predicted value (PI-c + MTT-m, PI-c + AUC-c + MTT-m) of DGF incidence were bigger than that of PI-c, AUC-c, or MTT-m.Conclusions-CEUS parameters of the cortex and medulla have a good value for an early prediction of DGF after renal transplantation.