The variation of the essential-oil composition among ten wild populations of Stachys lavandulifolia VAHL (Lamiaceae), collected from different geographical regions of Iran, was assessed by GC-FID and GC/MS analyses, and their intraspecific chemical variability was determined. Altogether, 49 compounds were identified in the oils, and a relatively high variation in their contents was found. The major compounds of the essential oils were myrcene (0.0-26.2%), limonene (0.0-24.5%), germacrene D (4.2-19.3%), bicyclogermacrene (1.6-18.0%), δ-cadinene (6.5-16.0%), pulegone (0.0-15.1%), (Z)-hex-3-enyl tiglate (0.0-15.1%), (E)-caryophyllene (0.0-12.9), α-zingiberene (0.2-12.2%), and spathulenol (1.6-11.1%). For the determination of the chemotypes and the chemical variability, the essential-oil components were subjected to cluster analysis (CA). The five different chemotypes characterized were Chemotype I (germacrene D/bicyclogermacrene), Chemotype II (germacrene D/spathulenol), Chemotype III (limonene/δ-cadinene), Chemotype IV (pulegone), and Chemotype V (α-zingiberene). The high chemical variation among the populations according to their geographical and bioclimatic distribution imposes that conservation strategies of populations should be made appropriately, taking into account these factors. The in situ and ex situ conservation strategies should concern all populations representing the different chemotypes.