Today, studying tourism discourse has become widespread among scholars in the field of text analysis. However, few, if any, studies which have addressed the language of tourism have examined the verbal content of travel brochures from the point of view of the appraisal model. The major questions addressed in this study pertain to Graduation strategies as part of appraisal strategies in the discourse of tourism as well as the lexico-grammatical resources for the coding of these strategies in texts. The dataset comprised 50 e-brochures released by tour operators across the United States within the period 2012 to 2013. First, the data were examined quantitatively to identify the statistical variations in utilizing Graduation strategies in tourist brochures. The preferences for lexico-grammatical resources for the construal of these strategies were also illustrated in light of a qualitative analysis. The results of the study revealed that the discourse of travel brochures is loaded with Graduation strategies. The subsystems within the system of Graduation were shown to serve as strong tools in promoting various aspects of tourist destinations such as the number and distribution of tourist sites over an area.

High-throughput single-cell genomics is used to gain insights into diseases such as cancer. Motivated by this important application, microfluidics has emerged as a key technology for developing comprehensive biochemical procedures for studying DNA, RNA, proteins, and many other cellular components. Recently, a hybrid microfluidic platform has been proposed to efficiently automate the analysis of a heterogeneous sequence of cells. In this design, a valve-based routing fabric based on transposers is used to label/barcode the target cells. However, the design proposed in prior work overlooked defects that are likely to occur during chip fabrication and system integration. We address the above limitation by investigating the fault tolerance of the valve-based routing fabric. We develop a theory of failure assessment and introduce a design technique for achieving fault tolerance. Simulation results show that the proposed method leads to a slight increase in the fabric size and decrease in cellanalysis throughput, but this is only a small price to pay for the added assurance of fault tolerance in the new design.

TWIK-related K+ 1 (TREK1) is a potassium channel expressed in the nervous system with multiple functions including neurotransmission and is a prime pharmacological target for neurological disorders. TREK1 gating is controlled by a wide range of external stimuli including mechanical forces. Previous work has demonstrated that TREK1 can be mechano-activated using magnetic nanoparticles (MNP) functionalised with antibodies targeted to TREK1 channels. Once the MNP are bound, external dynamic magnetic fields are used to generate forces on the TREK channel. This approach has been shown to drive cell differentiation in cells from multiple tissues. In this work we investigated the effect of MNP-mediated TREK1 mechano-activation on early stress response pathways along with the differentiation and connectivity of neuronal cells using the model neuronal cell line SH-SY5Y. Results showed that TREK1 is well expressed in SH-SY5Y and that TREK1-MNP initiate c-Myc/NF-κB stress response pathways as well as Nitrite production after magnetic stimulation, indicative of the cellular response to mechanical cues. Results also showed that TREK1 mechano-activation had no overall effect on neuronal morphology or expression of the neuronal marker βIII-Tubulin in Retinoic Acid (RA)/Brain-derived Neurotrophic factor (BDNF) differentiated SH-SY5Y but did increase neurite number. These results suggest that TREK1 is involved in cellular stress response signalling in neuronal cells, which leads to increased neurite production, but is not involved in regulating RA/BDNF mediated neuronal differentiation.

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