Background and objectives: Spontaneous abortion (SA), also known as miscarriage, is considered as one of the most frequent problems a woman may face during early pregnancy, is not only related to morbidity or mortality, but also has an obvious social and psychological impact on women. An abortion is the removal or expulsion of an embryo or fetus from the uterus, resulting in \or caused by its death, the loss of a pregnancy occurs within the first 20-24 weeks of gestation, after which, fetal death is known as a stillbirth. This study was first aimed to assess the frequency of, Cytomegalovirus, Rubella and Herpes simplex virus in embryonic tissues of women with abortion. Next to find out the sociodemographic characteristics of the studied populations. Methods: This cross-sectional prospective study was carried out from February 2019 through January 2020.This study prospectively deal with tissues from conceptus after abortion which collected from maternity teaching hospital in Erbil city, Iraq for the diagnosis of frequency of CMV, rubella and herpes simplex virus. RT-PCR were used in tissue analysis. SPSS version 25 was used for data entry and analysis. Results: Out of 72 cases with spontaneous abortion 8 (11.1%) were due to human Cytomegalovirus,2 (2.8%) were due to Rubella virus and 1case (1.4%) was due to Herpes simplex virus, the age range of participants was 17-45 years with mean ± Sd of (31.29 ± 6.11) years. The highest percentages of participants were in their second and third decades of life (40.5% and 37.5% respectively) and about 86.1% of them were unemployed (housewives) and majority about 57% of them attending primary school, and more than half percentage (63.8%) of them have intermediate economic state. Histopathological analysis shows that out of 72 cases only 9 samples (16.6%) show histopathological abnormalities. A statistically significant association was found between Rubella infection and histopathological abnormality. Conclusions: Cytomegalovirus infection was more prevalent among the study samples, followed by Rubella infection then Herpes simplex virus infection by real-time polymerase chain reaction, on other hand 16.6% of samples shown nonspecific histopathological abnormality.