Cedrela fissilis Vell. is a valuable wood species from Atlantic Forest and is at risk of extinction. Therefore, a protocol for clonal multiplication was developed for C. fissilis using different explants from in vitro seedlings. The seeds were germinated in total or half-strength MS and B5 basal media. The half-strength MS medium was chosen based on its high germination rate (82 %) and germination speed index (1.218). Shoot tips, nodal segments and cotyledonary nodes explants were extracted from the in vitro seedlings and cultivated for shoot induction in MS medium supplemented with KIN or BAP (0, 0.5, 2 and 4 μM). The highest number (2.2) and length (3.84 cm) of shoots were obtained from cotyledonary nodes cultured in the absence of cytokinin. For rooting, the MS medium was supplemented with IBA or NAA (0, 1 and 3 μM). Rooting was induced in all auxin treatments. The highest number of roots per explant was observed in the treatment supplemented with 3μM IBA (2.54). The present study provides in vitro shoot multiplication and root regeneration systems for C. fissilis, with great potential to be used for micropropagation, germplasm preservation and genetic transformation.