Me.thy.lo.te'ne.ra. N.L. neut. n. methylum the methyl group; N.L. pref. methylo‐ , pertaining to the methyl radical; L. fem. adj. tenera delicate; N.L. fem. n. Methylotenera , delicate, methyl‐utilizing organism. Proteobacteria / Betaproteobacteria / Nitrosomonadales / Methylophilaceae / Methylotenera The genus Methylotenera , part of the Methylophilaceae family, unites obligately and facultatively methylotrophic bacteria. Three species have been described: Methylotenera mobilis , Methylotenera versatilis , and Methylotenera oryzisoli . Cells of these methylotrophs are Gram‐negative rods or ovals that do not form resting bodies and reproduce by binary fission. Some strains are motile. All described genus members can use methylamine as a single source of carbon, energy, and nitrogen. The oxidation of methylamine has been demonstrated to occur via methylamine dehydrogenase or the N ‐methylglutamate pathway. Methanol utilization has been reported for most isolates. Methylotenera species use the pyrroloquinoline quinone‐dependent methanol dehydrogenase enzyme (XoxF), which requires rare earth elements as cofactors. The oxidation of methanol is often coupled with dissimilatory nitrate reduction. All tested strains use the ribulose monophosphate pathway for C1‐carbon assimilation. Some members are capable of multicarbon substrate utilization, such as fructose and pyruvate. Genomic evidences suggest the presence of the methylcitric acid cycle, while the Krebs cycle is incomplete. Nitrate and ammonia can serve as nitrogen sources. Some strains can use urea. Tests conducted for catalase and cytochrome oxidase are positive. The major fatty acids are C 16:1 ω7 c /C 16:1 ω6 c and C 16:0 . The genomes of 18 strains, representative of all three Methylotenera species, have been sequenced. Genome sizes range from 1.65 to 3.06 Mb, with a DNA G + C content of 41–50 (mol%). Methylotenera resides in a wide array of habitats, including polluted soil, marine and freshwater, acid mine drainage, terrestrial soils, and lake and glaciered sedimentary ecosystems. Type species : Methylotenera mobilis Kalyuzhnaya et al. 2006 VP .