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An immobilized glucose isomerase which is active and stable in concentrated glucose solution has been developed. The enzyme has been immobilized to Duolite A7 resin simply by adsorption. Industrial application of this immobilized enzyme for the continuous production of high fructose syrup is quite promising because the immobilized enzyme having enough mechanical strength against the pressure o f column flow, is active over broad ranges of both temperature and pH and is less dependent on M g and Coions.Long-run continuous experiments in column system have been carried out, isomerizing 41 "lo of glucose with flow rate of SV 1 at 6OoC. A gradual decrease of activity was observed affer 21 d operation and the half-life was calculated to be 36d. One liter of the immobilized enzyme in column isomerized 288 kg of glucose in a month. The values of kinetic parameters of the immobilized enzyme in column operation varied with flow rates and approached to those of the native enzyme when they were assayed at the flow rate approaching to SV 0. (Zusammenfassung Seite 306; Rlsuml d la page 306) Die Starke 27. lahrg. 1971 I N r . 9

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Both neutral lactase partially purified from yeast and acid lactase prepared from fungi were immobilized to weakly basic anion exchange resin (Duolite A7) chemically modified by a bifunctional reagent, 2-carboxymethylamino-4,6-dichloro-s-triazine. These immobilized enzymes could carry out a continuous hydrolysis of lactose in milk and whey, respectively, at appreciably high flow rates. The immobilized enzymes obtained were stable over broad ranges of pH and temperature and were protected from the attack by protease and bacteria.