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Seventy-six teeth from open canals were extracted to prepare serial longitudinal sections. The sections were made from apical portions of the teeth and stained. Ninety similar teeth were extracted to prepare dentinal splinters with files from the enlarged infected canal. The splinters were spread on a selected medium to grow S. mutans. S. mutans was detected in 48.7% of the 76 teeth examined. The distance of invasion of S. mutans in the dentinal tubules revealed by immunofluorescence averaged 509 micrometer from the canal wall and reached 1150 micrometer, depending on the serogroups of S. mutans. Unidentified germs in the sections which were demonstrated by Gram's stain invaded further than S. mutans. The frequency of appearance of the serogroups of S. mutans was 32.6% (d), 27.9% (c), 24.4% (a), and 15.5% (b).

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The immunosuppressive effects of cisplatin at relatively low doses were investigated in CD-1 mice. Mice were injected intraperitoneally with 8, 40 and 200 micrograms/kg cisplatin for 10 days. A decrease in body and thymus weights was observed at 200 micrograms/kg. Though there were no dose-related effects on the IgM antibody response to sheep erythrocytes, a statistically significant reduction of the contact hypersensitivity response (CHR) was seen at 200 micrograms/kg. In vivo and in vitro effects of cisplatin on T- and B-lymphocyte function were assessed by proliferative response to concanavalin A and lipopolysaccharide, respectively. Cisplatin inhibited splenic T-lymphocyte function more than splenic B-lymphocyte function. These data indicate that a relatively low dose of cisplatin induce immunosuppressive effects in mice with a greater effect on T-lymphocytes than the B-lymphocytes.

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