To understand plant molecular networks of glucosinolate metabolism, perturbation of aliphatic glucosinolate biosynthesis was established using inducible RNA interference (RNAi) in Arabidopsis. Two RNAi lines were chosen for examining global protein and metabolite changes using complementary proteomics and metabolomics approaches. Proteins involved in metabolism including photosynthesis and hormone metabolism, protein binding, energy, stress, and defense showed marked responses to glucosinolate perturbation. In parallel, metabolomics revealed major changes in the levels of amino acids, carbohydrates, peptides, and hormones. The metabolomics data were correlated with the proteomics results and revealed intimate molecular connections between cellular pathways/processes and glucosinolate metabolism. This study has provided an unprecedented view of the molecular networks of glucosinolate metabolism and laid a foundation towards rationale glucosinolate engineering for enhanced defense and quality.

This paper is concerned with the large-time behavior of solutions to the outflow problem of full compressible Navier-Stokes equations in the half line. This is one of the series of papers by the authors on the stability of nonlinear waves to the outflow problem. We show the time asymptotic stability of degenerate (transonic) stationary solution for the general gas including ideal polytropic gas. The key point of the proof is based the new property of the degenerate stationary solution and the delicate energy estimates.MSC 2010: 35Q30, 35B35, 35L65, 76D33, 74J40.

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