[1] Although boundary layer processes are important in climate, weather and air quality, boundary layer climatology has received little attention, partly for lack of observational data sets. We analyze boundary layer climatology over Europe and the continental U.S. using a measure of boundary layer height based on the bulk Richardson number. Seasonal and diurnal variations during 1981-2005 are estimated from radiosonde observations, a reanalysis that assimilates observations, and two contemporary climate models that do not. Data limitations in vertical profiles introduce height uncertainties that can exceed 50% for shallow boundary layers (<1 km) but are generally <20% for deeper boundary layers. Climatological heights are typically <1 km during daytime and <0.5 km at night over both regions. Seasonal patterns for daytime and nighttime differ; daytime heights are larger in summer than winter, but nighttime heights are larger in winter. The four data sets show similar patterns of spatial and seasonal variability but with biases that vary spatially, seasonally, and diurnally. Compared with radiosonde observations, the reanalysis and the climate models produce deeper layers due to difficulty simulating stable conditions. The higher-time-resolution reanalysis reveals the diurnal cycle in height, with maxima in the afternoon, and with amplitudes that vary seasonally (larger in summer) and regionally (larger over western U.S. and southern Europe). The lower-time-resolution radiosonde data and climate model simulations capture diurnal variations better over Europe than over the U.S., due to differences in local sampling times.

Surface-based inversions (SBIs) are frequent features of the Arctic and Antarctic atmospheric boundary layer. They influence vertical mixing of energy, moisture and pollutants, cloud formation, and surface ozone destruction. Their climatic variability is related to that of sea ice and planetary albedo, important factors in climate feedback mechanisms. However, climatological polar SBI properties have not been fully characterized nor have climate model simulations of SBIs been compared comprehensively to observations. Using 20 years of twice-daily observations from 39 Arctic and 6 Antarctic radiosonde stations, this study examines the spatial and temporal variability of three SBI characteristic-frequency of occurrence, depth (from the surface to the inversion top), and intensity (temperature difference over the SBI depth)-and relationships among them. In both polar regions, SBIs are more frequent, deeper, and stronger in winter and autumn than in summer and spring. In the Arctic, these tendencies increase from the Norwegian Sea eastward toward the East Siberian Sea, associated both with (seasonal and diurnal) variations in solar elevation angle at the standard radiosonde observation times and with differences between continental and maritime climates. Two state-of-the-art climate models and one reanalysis dataset show similar seasonal patterns and spatial distributions of SBI properties as the radiosonde observations, but with biases in their magnitudes that differ among the models and that are smaller in winter and autumn than in spring and summer. SBI frequency, depth, and intensity are positively correlated, both spatially and temporally, and all three are anticorrelated with surface temperature.

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The amyloid fibrils formed by heating 1.0% (w/v) kidney bean phaseolin (7S globulin) solution at pH 2.0 with an ionic strength of 20 mM at 85 degrees C were characterized using transmission electron microscopy (TEM), atomic force microscopy (AFM), binding of thioflavin T (Th T) and Congo Red dyes, and circular dichroism spectroscopy. The morphology of the formed fibrils was closely dependent upon heating time from 15 to 720 min. The diameters of the fibrils formed at various times were similar, but the mean contour length progressively increased with heating time. The Th T maximum fluorescence also progressively increased with heating time. The heating process caused remarkable changes in secondary, tertiary, and quaternary conformations of the phaseolin, but the extents of the changes were closely related to the heating time. With a short heating time (e.g., 15 min), the beta-strand content decreased from 38.7 to 22.9%, but further heating resulted in recovery of beta-strand structure. The tertiary and quaternary conformations gradually became flexible and unfolded upon heating. Gel electrophoresis analysis indicated that heating disrupted the polypeptides of phaseolin, leading to the formation of fragments with lower molecular mass (e.g., <10 kDa after 360 min). The results suggest that the amyloid fibril formation of phaseolin (7S globulin) involved the disruption of its polypeptides, as well as conformational changes at secondary, tertiary, and quaternary structural levels. This appears to be the first direct observation of amyloid fibrils from legume 7S storage globulin.

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