Objective To study the correlation between Traditional Chinese Medicine (TCM) constitution and dyslipidemia. Methods CNKI, VIP, Wanfang database, CBMdisc, PubMed, and Embase were searched, and meta-analysis was performed by Review Manager 5.2 software. Results Altogether 11 studies were included with 12890 individuals. The results showed that balanced constitution was a protective factor of dyslipidemia (OR = 0.62, 95% CI 0.47~0.82) while phlegm-dampness constitution was a risk factor of it (OR = 2.50, 95% CI 2.22~2.80), and the effect of phlegm-dampness constitution in South China (OR = 3.31, 95% CI 1.71~6.43) was more obvious than that in East (OR = 2.40, 95% CI 2.06~2.80) and North China (OR = 2.24, 95% CI 1.81~2.78). Conclusion This study provides evidence for the prevention and treatment of dyslipidemia in TCM. However, most of the studies included are of moderate quality; more high quality, multicenter, large-sample studies are expected to provide higher level evidence.

OBJECTIVES: Deaths due to opioid over-dosage have increased over the last decade and now represent the most frequent cause of death due to poisoning. This study examined the temporal association between prescription opioid use between 2000 and 2005 and the number of opioid-poisoning deaths. METHODS: The number of annual opioid-poisoning deaths from 2000-05 was obtained from the National Vital Statistics System multiple causes of injury mortality files (Warner 2009). Data for annual prescription opioid use was obtained from a published study that reported opioid use from 2.7 to 3.8 million enrollees annually using the HealthCore Blue Cross and Blue Shield national commercial insurance plans (Sullivan 2008). The temporal relationship between several opioid use measures and the number of opioid deaths/year was studied using ordinary least square regression. RESULTS: The cumulative opioid dose among all recipients increased from 204 mgs morphine equivalents/enrollee/year to 371 mgs and among opioid users with a non-cancer pain diagnosis from 2473 mgs to 3406 mgs. During this period, the number of yearly opioid deaths increased from 4,419 in 2000 to 10,947 in 2005. There was a significant linear relationship between the cumulative yearly opioid dose/enrollee/year and the number of opioid-poisoning deaths (pϽ0.0001, R 2 ϭ0.98). A significant positive linear relationship was also observed between the number of annual opioid-related deaths and opioid use measures of cumulative yearly opioid dose (pϽ0.0001, R 2 ϭ0.98), opioid dose/prescription (pϭ0.0053, R 2 ϭ0.88) and mean days-supplied of opioids/year (pϽ0.0001, R 2 ϭ0.99). CONCLUSIONS: A strong linear relationship between opioid-poisoning deaths and several prescription opioid use measures suggests that the acquisition of prescribed opioids through legal channels is associated with the national opioid mortality rate. This calls for added vigilance on the part of prescribers and policy makers to ensure safe use of opioid analgesics. Further research is warranted to confirm these correlations.

The formation and development of legumes nodules requires a lot of energy. Legumes must strictly control the number and activity of nodules to ensure efficient energy distribution. The AON system can limit the number of rhizobia infections and nodule numbers through the systemic signal pathway network that the aboveground and belowground parts participate in together. It can also promote the formation of nodules when plants are deficient in nitrogen. The currently known AON pathway includes four parts: soil NO3− signal and Rhizobium signal recognition and transmission, CLE-SUNN is the negative regulation pathway, CEP-CRA2 is the positive regulation pathway and the miR2111/TML module regulates nodule formation and development. In order to ensure the biological function of this important approach, plants use a variety of plant hormones, polypeptides, receptor kinases, transcription factors and miRNAs for signal transmission and transcriptional regulation. This review summarizes and discusses the research progress of the AON pathway in Legume nodule development.

Objective. To observe the effects of Asini Corii Colla, turtle carapace glue, and other drugs on the intestinal flora of nude mice with uterine fibroids model, so as to provide evidence for the clinical application of drugs. Methods. Set up five groups: blank control group, turtle carapace glue group, turtle carapace glue and ejiao 4 : 1 mixed group, turtle carapace glue and ejiao 1 : 1 mixed group, and turtle shell glue and Salvia miltiorrhiza (danshen) 1 : 1 mixed group. Then, the model nude mice were fed ejiao, turtle carapace glue, and other corresponding drugs. Before administration, 2 weeks after administration, and 4 weeks after administration, the feces of the model nude mice were taken respectively, subpacked into labeled cryotubes, and stored at −80°C. All samples were sent for gene sequencing after completion. The differences in gut microbiota and abundance in different groups were compared by 16SrRNA segment sequencing. Results. ① There were differences in flora composition and a relative abundance among the groups, but the strains with a high relative abundance were Bacteroides, Firmicutes, and Proteobacteria; ② there were significant differences in the community structure and composition of intestinal flora between nude mice treated for 4 weeks and those not treated ( p < 0.05 ); ③ after 4 weeks of administration, the relative abundance of Bacteroidetes in each group was higher than that before administration, and the relative abundance of Firmicutes decreased. Conclusion. Asini Corii Colla, turtle carapace glue, and other drugs with different compatibility ratios can change the composition of intestinal flora in nude mice with uterine fibroids to a certain extent; the decrease in the relative abundance of Firmicutes and the increase in the relative abundance of Bacteroidetes were important structural changes of intestinal flora in nude mice at 4 weeks after administration.

Medicago truncatula has been selected as one of the model legume species for gene functional studies. To elucidate the functions of the very large number of genes present in plant genomes, genetic mutant resources are very useful and necessary tools. Fast Neutron (FN) mutagenesis is effective in inducing deletion mutations in genomes of diverse species. Through this method, we have generated a large mutant resource in M. truncatula. This mutant resources have been used to screen for different mutant using a forward genetics methods. We have isolated and identified a large amount of symbiotic nitrogen fixation (SNF) deficiency mutants. Here, we describe the detail procedures that are being used to characterize symbiotic mutants in M. truncatula. In recent years, whole genome sequencing has been used to speed up and scale up the deletion identification in the mutant. Using this method, we have successfully isolated a SNF defective mutant FN007 and identified that it has a large segment deletion on chromosome 3. The causal deletion in the mutant was confirmed by tail PCR amplication and sequencing. Our results illustrate the utility of whole genome sequencing analysis in the characterization of FN induced deletion mutants for gene discovery and functional studies in the M. truncatula. It is expected to improve our understanding of molecular mechanisms underlying symbiotic nitrogen fixation in legume plants to a great extent.