The object of this study was to reduce the alginate content of Sargassum binderi by fermentation of Bacillus megaterium S245 in feed of laying hens. The experiment was arranged in a factorial with completely randomized design. The first factor was different inoculum dosage (1, 3, 5, 7, and 9%), while the second factor was different fermentation period (1, 3, 5, 7 and 9 days), and each treatment was replicated for five times. The measurements were total dry matter, organic matter, ash, alginate, and crude protein. The results showed there were no significant effect of inoculum dosage, and interaction between inoculum dosage and fermentation period on alginate, total dry matter, organic matter, ash and crude protein content of Sargassum binderi while fermentation period reduced the alginate and total dry matter content. Fermentation period also increased the crude protein content significantly. Besides that, fermentation period didn't effect on organic matter and ash content significantly. The fermentation of Sargassum binderi with Bacillus megaterium S245 at inoculum dosage of 1% and fermentation period of nine days was the best combination for lowering alginate content in Sargassum binderi and this treatment had positive effect on nutrient content of Sargassum binderi.

Corn straw and cob are wastes from corn farming which are not utilized. These corn wastes have potential as an alternative feed for livestock, but have low nutritional quality. Therefore, these corn wastes are fermented to improve its nutritional quality. The purpose of this study was to analyze the effect of corn straw and cob fermentation on the fiber fraction content. This experiment used a completely randomized design with 6 treatments, namely control (elephant grass and grinting grass), corn straw, fermented corn straw, corn cob, and fermented corn cob. Each treatment was repeated 5 times. The variables measured were Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), hemicellulose, cellulose, and lignin. The results showed that the treatment had a very significant effect on the content of NDF, ADF, hemicellulose, cellulose, and lignin. Based on the results of this experiment, it can be concluded that corn cob fermentation can reduce lignin content thus corn cob lignin content can match elephant grass but on the contrary corn straw fermentation cannot be able to reduce lignin content.

Sargassum binderi has been potentially used as laying hen feed since it contains bioactive compounds useful for poultry health. In addition, the high alginate content of S. binderi has made it inappropriate for the poultry diet. Therefore, the alginate content should be reduced before its use in poultry feed. This study aimed to reduce the alginate of S. binderi for use as laying hen feed. The experiment was performed in two phases in a completely randomized design. The first phase included heated S. binderi in the autoclave and the second phase entailed the immersion of S. binderi in whiting filtrate. The treatments in the physical method contained a control group, and four treatment groups heating for 15, 30, 45, and 60 minutes. The treatments in the chemical method had a control group and four treatment groups with immersion periods of 1, 2, 3, and 4 hours. Each treatment was repeated five times, and the investigated parameters were crude protein, total dry matter, organic matter, ash, and alginate, respectively. The heating durations of S. binderi in an autoclave and different immersion periods of S. binderi in whiting filtrate did not significantly affect total dry matter, organic matter, ash, alginate, and crude protein. The results of this study showed that physical treatment (heat treatment) and chemical treatments (whiting filtrate immersion) did not have a significant effect on the alginate content, crude protein, ash, dry matter, and organic matter. Keywords: Alginate, Heating, Laying hen, Sargassum binderi, Whiting filtrate