Aim: To evaluate the antimicrobial potential of irreversible hydrocolloid impression material manipulated using chitosan impregnated solution at various time intervals. Setting and Design: Evaluative invivo study design. Materials and Methods: Maxillary impressions made for 20 dentulous volunteers using irreversible hydrocolloid impression material manipulated using distilled water as control and using 1% chitosan impregnated solution as test group using stock metal trays with one-week interval. Bacterial samples were collected using dry sterile cotton swab in the mid-palatal region at the time intervals of 0, 10, 20 minutes. Bacterial swabs were inoculated on nutrient agar media and incubated at 37° C for 24 hours. Bacterial colonies were counted with the aid of colony counter. Statistical Analysis Used: The resultant data was subjected to statistical analysis using repeated measures ANOVA and independent t test. Results: Adding water soluble chitosan to irreversible hydrocolloid impression material resulted in superior antimicrobial activity. With the passage of time there was a significant decrease in the microbial colony count upto 10min (p=0.016). However, the rate of decrease of microbial colony count was statistically insignificant between the samples collected at 10 and 20 min. Conclusion: Incorporation of water soluble chitosan to irreversible hydrocolloid impression material showed significant antimicrobial activity in 10 minutes.

Aim: To evaluate the changes in temporomandibular joint (TMJ) vibrations after insertion of the complete denture in edentulous patients. Settings and Design: An observational in vivo study conducted to evaluate the changes in maximum mouth opening, total integrals, peak amplitude, and peak frequency in TMJ vibrations on the day of complete denture insertion and 6 months of follow up. Materials and Methods: Twenty patients (male: 12 and female: 8) were selected for the fabrication of balanced complete dentures following conventional procedure. Joint vibration analysis was recorded using the joint vibration analyzer. The patients were instructed to open as wide as possible and close to the intercuspal position with rhythmic speed following the metronome projected on the screen. The TMJ vibrations were amplified and displayed as waveforms in a graphical representation by system software. Statistical Analysis Used: Wilcoxon test. Results: A significant decrease in the total integral ( P = 0.001) and peak amplitude ( P = 0.044) for opening and closing movements of the left and right joints was observed. There was no significant change in maximum mouth opening ( P = 0.624). A decrease in peak frequency was noted only at left opening movements between the day of insertion and at 6 months ( P = 0.025). Conclusion: The function of TMJ mechanics was improved till 6 months after insertion of complete denture with balanced articulation (BA).