Abstract. A bilayer hybrid nanoimprinting (NI) method was developed for fabricating embedded metal nanopatterns with greater processability and improved reliability for enhanced photoluminescence (PL) in optoelectronic devices. Bilayer hybrid NI consists of the following: (a) spin-coating ultraviolet (UV) and thermally curable NI resists in sequence, (b) high-pressure thermal NI and UV exposure while maintaining the stamp in a pressed position, and (c) silver (Ag) deposition and lift-off using a thermal NI resist on the upper surface to create embedded Ag nanoarrays. Reference samples with no Ag nanopatterns and with protruding Ag dot-shaped nanopatterns were also fabricated for comparison. The transmittance and PL of all samples were measured. All samples containing Ag nanopatterns exhibited improved PL compared with reference samples with no Ag. For all pattern sizes, the samples with the embedded Ag nanoarrays exhibited the highest PL; the relative PL enhancements compared with samples with Ag dot-shaped nanoarrays were 32.2%, 36.1%, and 62.7% for pattern sizes of 150, 200, and 265 nm, respectively.

Clinical cases of type-1 hypersensitive reaction to rice (Oryza sativa) have been reported in western countries as well as in Japan. Among rice proteins, 14-16 kD globulin proteins encoded by multiple gene family have been identified as major rice allergens. In this study, a rice cDNA library was constructed using λ λ λ λ UniZap vector and screened with a rat anti-16 kD globulin protein polyclonal antibody in order to isolate Korean rice allergenic cDNA clones. Five independent cDNA clones, termed RAK1-5, were obtained after second rounds of plaque assay and immunoblot analysis. These clones encoded 13-19 kD recombinant proteins upon IPTG induction, which were identified by the polyclonal antibody in immunoblot analysis. DNA sequencing analysis showed that RAK1-4 have 99% sequence homology with RA5b, and RAK5 is closely related with RA14c. This result indicated that RA5b gene is widely distributed in our cDNA library among other possible rice allergenic genes, and more study is needed to isolate heterogeneous or novel rice allergen genes.

Despite substantial progress in metal nanopatterning, fabricating ultra-large-area plasmonic substrates with well-defined and well-controlled nanopatterned arrays remains a major technological challenge. Here, we describe a novel facile technology (i.e., configurable metal nanoimprint transfer based on geometric reconfiguration during thermal annealing) to fabricate ultra-large-area tunable plasmonic substrates. The simultaneous transfer and imprint of the metal layers from the patterned mold surface results in metal nanopatterns embedded in a partially cured photoresist, the shape of which can be modified systematically by optimized heat treatments. The plasmonic properties of the metal nanopattern array could be precisely tuned through the heat-driven shape reconfiguration of metal patterns. The shape transformation leads to sharp and blue-shifted extinction spectra and unusual strong excitation of the transverse mode of metal nanopatterns. Coarse tuning of the plasmon resonance wavelength is achieved by varying the diameter of the nanopatterned features, and fine tuning is accomplished by reconfiguring the geometry of the nanopatterned features via thermal annealing. Only three master patterns are required to cover the wavelength range 535-837 nm. By applying the plasmon substrates to photoluminescence (PL) measurements, an enhancement in the green photoluminescence (PL) intensity of a factor more than 9.4 is achieved due to the improved matching between the wavelengths for PL emission and plasmon resonance. The fabrication strategy described here enables us to achieve various plasmonic properties using a single master pattern, which provides both tailorable plasmonic properties and remarkable process flexibility.

Objectives:The purpose of the study is to investigate the influencing factors of community scaling rate using community health survey data.

Using CpGIE(CpG Island Explorer), we evaluated suggested criteria for the search of CpG island of human gene chromosome 18, 19, 20. Manipulating three different criteria: length, %GC, and ObsCpG/ExpCpG rate, we found most appropriate criteria for CpG detection. One of the most important processes of our assessment is to decide whether the new criteria effectively exclude Alu repeats. Moreover, by assorting association type: promoter-relation, within-relation, end-relation, we confirmed that CpG islands defined by the new criteria showed better function as gene markers.