Abstract.Aim: This purpose of research is to ascertain nurses' knowledge level and attitudes about the sexuality of the elderly. Background: This research is a descriptive study. The objects were 231 nurses who have been working at general hospitals and long-term care hospitals in S, I City. Method: The data were analyzed with SPSS version 15.0. Frequency, percentage, average & standard deviation, t-test, ANOVA and Duncan method as a post-hoc were used. Result: The mean of the level of knowledge and attitude of nurses regarding sexuality of the elderly was 18.97±5.58, and 39.03±5.18 respectively. Conclusion: It is thought that efforts should be done not only on strengthening the academic curriculum of nursing related to the sexuality of the elderly at the school as nursing students university, but also on developing and applying the continuous systematic education module according to the level of the clinical nurses caring the elders by continuing education for improving their knowledge and attitude toward sexuality of the elderly.