In view of the looming energy crisis as a result of depleting fossil fuel resources and environmental concerns from greenhouse gas emissions, the need for sustainable energy sources has secured global attention. Research is currently focused towards renewable sources of energy due to their availability and environmental friendliness. Biofuel production like other bioprocesses is controlled by several process parameters including pH, temperature and substrate concentration; however, the improvement of biofuel production requires a robust process model that accurately relates the effect of input variables to the process output. Artificial neural networks (ANNs) have emerged as a tool for modelling complex, non-linear processes. ANNs are applied in the prediction of various processes; they are useful for virtual experimentations and can potentially enhance bioprocess research and development. In this study, recent findings on the application of ANN for the modelling and optimization of biohydrogen, biogas, biodiesel, microbial fuel cell technology and bioethanol are reviewed. In addition, comparative studies on the modelling efficiency of ANN and other techniques such as the response surface methodology are briefly discussed. The review highlights the efficiency of ANNs as a modelling and optimization tool in biofuel process development.

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