AbstrakBakteri vagina adalah sumber utama dari laktat asam di vagina. Lactobacillus berperan memberikan proteksi pada sel epitelial vagina dengan memberikan bantuan pada hubungan antar sel serta sekresi bakteriosida berupa Hidrogen Perioksida (H2O2). Lactobacillus sebagai mikroflora dominan vagina mampu menjaga pH vagina 4,5 sehingga dapat mengurangi risiko kolonisasi bakteri patogen. Peningkatan pH vagina menguntungkan bagi kelangsungan hidup Lactobacillus dan karakteristik Lactobacillus sebagai produk probiotik vagina. Penelitian ini bersifat deskriptif bertujuan mengetahui jenis bakteri asam laktat yang diisolasi dari vagina wanita usia subur dengan pemeriksaan makroskopis, mikroskopis, dan karakteristik molekuler. Populasi dari penelitian ini adalah wanita usia subur. Sampel penelitian ini sebanyak 39 orang. Teknik pengambilan sampel adalah consecutive sampling. Hasil penelitian didapatkan rata-rata umur responden 32 tahun, kelompok umur terbanyak 25-30 tahun (79,4%), rata-rata paritas responden adalah 2 orang, dan 2 diantara responden adalah wanita hamil. Dari 39 sampel setelah dilakukan pewarnaan Gram, didapatkan hampir semua isolat bakteri adalah bakteri Gram positif dengan bentuk kokus dan basil. Berdasarkan hasil identifikasi molekuler dengan analisis sekuensing didapatkan jenis Bakteri Asam Laktat, Lactobacillus crispatus, Lactobacillus oris, Lactobacillus salivarius, dan Enterococcus faecalis. Abstract Vaginal bacteria are the main source of lactic acid in the vagina. Lactobacillus acts to provide protection to vaginal epithelial cells by providing assistance in the intercellular relationship and bacteriocide secretion in the form of Hydrogen Perioxide (H2O2). Lactobacillus as the dominant vaginal microflora is able to maintain a vaginal pH of 4.5 so as to reduce the risk of pathogenic bacterial colonization. Increased vaginal pH is beneficial for the survival ofLactobacillus and the characteristics of Lactobacillus as a vaginal probiotic product. This research is descriptive in nature to find out the types of lactic acid bacteria isolated from the vagina of women of childbearing age by macroscopic, microscopic, and molecular characteristics. The population of this study were reproductive age women. The sample of this study was 39 people. The sampling technique was consecutive sampling. The results showed that the average age of respondents was 32 years, the highest age group was 25-30 years (79.4%), the average parity of respondents was 2 people, and 2 of the respondents were pregnant women. Of the 39 samples after Gram staining, almost all bacterial isolates were Gram positive bacteria with coccus and bacillary forms. Based on the results of molecular identification by sequencing analysis, Lactobacillus crispatus, Lactobacillus oris, Lactobacillus salivarius, and Enterococcus faecalis were found.