Work that requires being in a humid area, can cause fungal infections. Skin fungal infections in humans can be divided into two, namely dermatophytosis and non-dermatophytosis. Dermatophytosis is a superficial fungal infection that attacks the keratinized parts of the skin, nails and hair and is caused by 3 genera, namely Microsporum, Epidermophyton, and Trichophyton. While non dermatophytosis is a fungal infection of the outer skin or this type of fungal infection does not reach the keratin tissue. Non-dermatophyte fungi include Aspergillus sp., Malessezia furfur. Due to dermatophyte and non-dermatophyte fungal infections, namely itching of the skin or earlobe. This activity aims to increase the understanding of cleaning workers at one of the universities in Surabaya about the dangers of fungus on the skin, so that they can prevent and anticipate early spread. The method used in this community service activity is the presentation and discussion of the dangers of skin fungal infections. To measure the level of understanding of the participants regarding the dangers of toxoplasmosis, a pretest was conducted at the beginning and at the end of the activity. This activity was attended by 22 janitors, both male and female. The average pretest score is 49,63 points, while the average posttest score is 71,48 points. These results indicate that there is an increase in the knowledge of cleaning workers at one of the universities in Surabaya about the causes, dangers, modes of transmission and methods of preventing skin fungal diseases. Educational activities are expected to be sustainable so that the community can apply steps to prevent skin fungal infections.

ABSTRAKIndonesia merupakan salah satu Negara berkembang dengan kasus kecacingan tinggi. Kasus kecacingan ini disebabkan oleh infeksi cacing usus yang penularannya melalui tanah (Soil Transmitted helmint). Prevalensi di Indonesia terutama pada anak usia sekolah dasar sebesar 60%-80%.  Cacing yang menjadi penyebab utama infeksi adalah Ascaris lumbricoides, Trichuris trichiura dan cacing tambang. Infeksi cacing ini dapat menyebabkan gangguan pencernaan, penyerapan serta metabolisme makanan sehingga protein, karbohidrat, lemak, vitamin dan darah dapat hilang dalam jumlah yang banyak. Dampak terburuk adalah  menurunnya plasma insulin like growth factor, peningkatan kadar serum tumor necrosis factor a (TNF), dan konsentrasi hemoglobin rerata semakin menurun. Eosin 2% merupakan pewarna yang selama ini digunakan dalam pewarnaan telur cacing nematode usus. Batang pohon jati (Tectona grandis)  mengandung pigmen pewarna alami seperti beta karoten dan turunan antosianin yang mampu memberikan warna merah, biru, orange, atau keunguan. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan rendaman batang pohon jati (Tectona grandis) sebagai pewarna alami pengganti Eosin untuk mewarnai telur cacing usus ((Soil Transmitted helmint). Penelitian dilakukan secara eksperimen dengan perlakuan batang pohon jati direndam selama 24 jam dalam alkohol 96% dan Eosin sebagai kontrol positif. Kemudian perlakuan digunakan untuk memeriksa feses yang diberi darah dan tanpa diberi darah. Hasil penelitian menunjukkan bahwa batang pohon jati yang direndam selama 24 jam dapat dijadikan alternatif pengganti reagen Eosin 2% untuk mewarnai telur cacing pada kedua sampel pemeriksaan (feses yang diberi darah dan tanpa darah). Hal ini dapat disimpulkan bahwa rendaman batang pohon jati dapat digunakan sebagai pewarna alami telur cacing.

Background and Objectives: In 2020 the whole world is experiencing a pandemic condition due to infection with the SARS-CoV-2 virus which can cause the COVID-19 disease. This condition results in “Panic Buying”, because everyone tries to avoid the spread and transmission of the COVID-19 disease by doing various ways, one of which is by taking additional supplements such as vitamin C and probiotic supplements. Materials and Methods: The materials used were mice Mus musculus male DDY strain aged 1-2 months. Probiotic supplement Lactobacillus acidophilus La-14 with a viability of 1 × 108 CFU/ml. with a weight of 0.16 grams dissolved in 0.25 ml 0.9% NaCl. Vitamin C used is a commercial vitamin C tablet, weighing 0.06 grams in 0.25 ml 0.9% NaCl. Meanwhile, the feed for mice (Mus musculus) is a complete feed from Pokphand with the code BR1 CP511B. Lung histology preparations data were analysed descriptively and statistically through the test Chi square while the data on the number of lymphocytes were analysed descriptively. Results: The histological observations of lungs of Mus musculus showed that in the treatment of ML, MV, and MKA test was carried out chi square ratio between the groups that did not have lymphocyte infiltration and those that had lymphocyte infiltration showed a significant difference (p <0.05). Meanwhile, the results of the lymphocyte count showed that ML and MV treatment was higher than that of MK treatment. Conclusion: It is suggested that the administration of probiotics can stimulate and modulate the respiratory immune system.

Infectious diseases caused by Staphylococcus aureus are a health problem in Indonesia. Antibiotics can be used as the treatment, but currently Staphylococcus aureus is resistant to antibiotics. Therefore, it is necessary to find alternative medicine. One of the plants that has characteristics to be used as herbal medicine is Breadfruit leaves. The purpose of this study was to determine the effect of concentration on breadfruit leaf juice which can inhibit the growth of Staphylococcus aureus bacteria. This type of research is experimental. The study population was pure Staphylococcus aureus bacteria on NAS (Nutrient Agar Slant) media. The sample in this study was pure Staphylococcus aureus transferred from pure culture media (NAS), while the number of repetitions of the sample was 5 times the treatment, in this study were P1 (100%), P2 (75%), P3 (50%), P4 (25%), P5(0%). Data on the growth of Staphylococcus aureus bacteria was obtained by indirect observation, namely through laboratory tests and data collection techniques using the ANOVA test with an error rate (0.05) and followed by the Tukkey HSD test through the SPSS16.0 program. Based on the results of the antibacterial test of breadfruit leaf juice (Artocarpus altilis) in the ANOVA test, the probability number was 0.000 and there was an effect on the growth of Staphylococcus aureus bacteria. Followed by the Tukkey HSD test, it was obtained at a concentration of 25% which is the minimum inhibitory power, the effective concentration to inhibit the growth of Staphylococcus aureus at a concentration of 75%. From the data above, it can be concluded that there is an effect of breadfruit leaf juice (Artocarpus altilis) on the growth of Staphylococcus aureus.©2020 JNSMR UIN Walisongo. All rights reserved.

Background:  Amelogenin gene was a single copy gene located in an X chromosome and a Y chromosome. The location of amelogenin gene for identification of sex chromosome has good variability between the form and the shape of the X chromosome and the Y chromosome and between Amelogenin alleles among different populations. Purpose: To prove urine spot examination on the results of the sex determination through Deoxyribo Nucleid Acid (DNA) isolation using amelogenin and Y chromosome loci (DYS19). Methods: Spotting the microscopic examination of urine samples to determine the presence or absence of urethral epithelial cells, followed by isolation Deoxyribo nucleid Acid (DNA) in order to determine the extent and purity of DNA amplification. Then performed Polymerase Chain Reaction (PCR) amelogenin locus at 106bp - 112bp and Y chromosomes (DYS19) at 232 -268 bp. Results: in 9 samples of men from 3 families with 3 kinship of different regions shows the results of different tests, because Amel Y variation between individual and populations method of determining the sex of 100% was inaccurate. In some men Amel Y can be removed entirely. This research should be visualized one band on the Y chromosome (DYS19) and the Amelogenin two bands during electrophoresis occurs misidentification of the sample as a woman. Conclusions: Identification of sex using Amelogenin locus and Y chromosomes (DYS19) has six identical and ambiguous results because the two samples shown as the sign of men but visualized as women, another sample was not visualized because of the thick level and concentration of Deoxyribo nucleid Acid (DNA).Keywords: Urine Spot, Sex Determination, Amelogenin, Y chromosome (DYS19).