Introduction: Osteoporotic fracture is a major public health problem in the pre and peri-menopausal period, associated with substantial morbidity, socio-economic burden and mortality. Poor knowledge and practice related to osteoporosis have been noted worldwide. In Malaysia, few studies have measured the knowledge and practice of osteoporosis among women. Objectives: This study aimed to determine the knowledge and practices of osteoporosis preventive measures among women in Hospital USM. Methods: A cross-sectional study using a validated, self-administered questionnaire about knowledge and practices of osteoporosis measures was employed with 185 hospitalized women in Hospital USM. A simple random sampling was used for the recruitment of participants. The data were analyzed using Chi-Square tests. Results: The Majority of the participants, 67%, belong to 45 -55 age group with a mean age of 50.35 years. The findings indicate 40.5% had a good level of knowledge towards osteoporosis, while 53.5% had a poor practice level. Conclusion: Knowledge of osteoporosis and practices is still at a fair level, affecting osteoporosis prevention practices. Time was spend watching television. Therefore, public health strategies should also be developed in Malaysia to increase women's knowledge of osteoporosis prevention and change lifestyle habits.