Filamin C (FLN c) is a novel allergen in shellfish. In this study, FLN c from Scylla paramamosain was divided into three regions for recombinant expression based on the number of domains and amino acids. Using dot blot and basophil activation tests, the allergic predominant region of FLN c was determined to be 336−531 amino acid positions (named FLN c-M). It was confirmed that by X-ray diffraction, the crystal structure of FLN c-M with immunoglobulin-like folding at a resolution of 1.7 Å was obtained. The monomer was a barrel structure composed of 16 β-strands and 2 α-helices. Three conformational epitopes were predicted, six linear epitopes were verified by serological test, and they were positioned on the crystal structure of FLN c-M. For the first time, the crystal structure of the allergic predominant region of FLN c was determined, and it provided an accurate template for the localization of IgE epitopes.

Objective: Interpersonal theories of suicide suggest that the Interpersonal Needs Questionnaire (INQ) can be used to measure suicidal ideation, but few studies have focused on migrant people, a group with a high prevalence of suicidal ideation. The aim of this study was to validate the psychometric properties of the INQ-15 and its prediction of suicidal ideation among migrant industrial workers in China. Method: A stratified multi-stage sample of 2023 industrial workers was recruited from 16 factories in Shenzhen, China. There were 1805 nonlocal workers, which we defined as migrant workers with a mean age of 32.50 ± 8.43 years old who were 67.3% male. The structure of the Chinese version of the INQ-15 and its correlation and predictive utility for suicidal ideation were examined through factor analysis, the Item Response Theory, the M2 test, logistic regression, and receiver operating characteristic (ROC) analysis. Results: Different from studies among various samples in which a two-factor solution is identified, results within this sample indicated three factors: perceived burdensomeness, thwarted belongingness, and social isolation. The model fit statistics of three-factor INQ were 0.075 for RMSEA, 0.945 for CFI, 0.932 for TLI, and 0.067 for SRMR. The model standard estimated factor loadings ranged from 0.366 to 0.869. The summed scores of INQ and perceived burdensomeness predicted suicidal ideation after controlling for sociodemographic characteristics such as age, gender, and income with AUC of 0.733 (95% CI: 0.712/0.754) and 0.786 (95% CI: 0.766/0.804). In the meantime, the comparison of the predictive ability between INQ total scores and PB scores was significant with p < 0.05. Conclusion: The INQ has good psychometric properties and can be used to assess how migrant workers living in the Shenzhen perceive meeting interpersonal psychological needs and shows good predictive ability of suicidal ideation. Perceived burdensomeness appears to play a role in suicide and may be a point of intervention, yet the notable deviation from previous findings and the relative weakness of two of the other factors warrant further study.

To improve the haptic sensing performance of electronic skin (e-skin), this study designed a capacitance-based highly sensitive three dimensional (3D) force tactile sensor with an inverted pyramidal structure with high electrical stability and mechanical repeatability. The working mechanism of the sensor was verified by finite element simulation, and it was fabricated by low-cost 3D printing technology and layer-by-layer (LBL) self-assembly process. A capacitive signal acquisition system and an application test platform were constructed. The results revealed that the proposed 3D-force tactile sensor had a normal force sensitivity of 0.551 N-1 at 0-7 N and 0.107 N-1 at 7-35 N. The results for tangential force were 0.404 N-1 at 0-4 N and 0.227 N-1 at 4-14 N, with a low hysteresis of 4.17% and a fast response/recovery time of 56/30 ms. High sensitivity and reliability of the device were demonstrated experimentally. The proposed capacitive flexible 3D-force haptic sensor can be used in applications such as robotic gripping, gamepad control and human motion detection, and its feasibility for application as e-skin was confirmed.

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C-axis-oriented YBa,Cu,O,., films produced by electron beam and thermal evaporation are composed of mosaic blocks. The deoxygenated YBa,Cu,O, (x = 6 . 1 4 2 ) films were annealed in an oxygen atmosphere. The change of oxygen concentration in t h e films during t h e annealing process was detected by the diffraction peak site of the (00 il) peak. Applying t h e theory of lattice diffusion to analyse the oxygen indiffusion in the'films. w e obtain the activation energiesfoi oxygendiffusioninthetetragonalandorthorhombicphases.whichare1.15and 1.23eV a coexistent range of two phases near t h e transition point. The difference behveen single crystals and c-axis film is that there are many mosaic boundaries in the c-axis film. A mosaic boundary acts as a short-circuit for oxygen diffusion and makes t h e diffusion time, during which equilibrium oxygen concentration is attained in the films. decrease greatly