Regular exercise has been shown to benefit its practitioners and prevent and control diseases. Muscle/Tendon Change Classic (MTCC) qigong, characterized by simple, slow, and full-body exercise, is appropriate for the middle-age population. This study aims to evaluate the effect of the MTCC qigong program in improving physical status for middle-aged women. A quasi-experimental design was used. The experimental group (n = 37) received an 8-week MTCC qigong program, whereas the control group (n = 34) received none. Physiological parameters of muscular performance, body composition, and bone strength were measured before and after the program. The average age was 49 +/- 4.13 years for the experimental group and 50 +/- 4.74 years for the control group. The demographic characteristics were homogeneous between the two groups. There were statistically significant differences between the two groups in muscular endurance, body fat, waist-to-hip ratio, and body mass index at the completion of 8-week MTCC qigong program. The MTCC qigong could improve muscle endurance and body composition but not bone strength for middle-aged women, thereby demonstrating the qigong practice has certain health-preserving effects on women in this stage of life.