We reported the complete chloroplast genome of Pyrrosia calvata, a traditional Chinese medicinal fern only restricted to Guangxi, China. Its genome is 158,201 bp in length with 41.59% GC content and includes two inverted repeats (IRs) of 26,983 bp separated by LSC and SSC of 82,517 bp and 21,718 bp, respectively. A total of 131 genes are predicted, including 88 protein-coding genes, 34 tRNA genes, eight rRNA genes, and one pseudogene. ML tree indicates that P. calvata is sister to Lepisorus clathratu. This study provides a new perspective to explore the phylogenetic relationship of Pyrrosia.

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