Coronavirus disease (COVID-19) pandemic is an unprecedented challenging time for parents and adolescents. The present study examines the role of parent work-life conflict on adolescent adjustment (i.e., academic engagement and mental health) and family processes (i.e., parental mental health and parenting) as potential mediators for this association. A total of 692 middle school students (53.2% boys; M age = 13.54 years, SD age = 0.58) and their parents (29.6% fathers and 70.4% mothers; M age = 44.75 years, SD age = 4.14 years) completed an online survey in May 2020 in Beijing, China. Results indicated that many parents (24.6%) experienced work-life conflicts amid the COVID-19 pandemic. Findings also showed that parent work-life conflict was negatively associated with youth academic engagement and mental health indirectly through parental mental health difficulties and parenting behavior (parental control, autonomy granting, and parental involvement). In addition, parental mental health difficulties had direct and indirect effects on youth adjustment via parenting behaviors, such that parental involvement and autonomy granting predicted greater academic engagement and covitality (co-occurrence of positive traits and positive mental health), whereas the parental control predicted youth mental health difficulties. Our findings extend prior research by examining the pathways linking parental work-life conflict to youth adjustment during COVID-19. Findings are discussed in terms of how to better support families and promote better youth academic engagement and well-being during COVID-19.
Trichomes, which develop from epidermal cells, are regarded as one of the key features that are involved in the evaluation of tea quality and tea germplasm resources. The metabolites from trichomes have been well characterized in tea products. However, little is known regarding the metabolites in fresh tea trichomes and the molecular differences in trichomes and tea leaves per se. In this study, we developed a method to collect trichomes from tea plant tender shoots, and their main secondary metabolites, including catechins, caffeine, amino acids, and aroma compounds, were determined. We found that the majority of these compounds were significantly less abundant in trichomes than in tea leaves. RNA-Seq was used to investigate the differences in the molecular regulatory mechanism between trichomes and leaves to gain further insight into the differences in trichomes and tea leaves. In total, 52.96 Gb of clean data were generated, and 6560 differentially expressed genes (DEGs), including 4471 upregulated and 2089 downregulated genes, were identified in the trichomes vs. leaves comparison. Notably, the structural genes of the major metabolite biosynthesis pathways, transcription factors, and other key DEGs were identified and comparatively analyzed between trichomes and leaves, while trichome-specific genes were also identified. Our results provide new insights into the differences between tea trichomes and leaves at the metabolic and transcriptomic levels, and open up new doors to further recognize and re-evaluate the role of trichomes in tea quality formation and tea plant growth and development.
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