We have studied the potential of saturation transfer nuclear magnetic resonance (NMR) experiments in different mode to screen compound mixtures for binding activity and to characterize binding epitopes on the ligand. We have developed a protocol based on the transfer of saturation from the protein to bound ligands which by dissociation is moved into solution where it is detected (Fig. 1; Mayer and Meyer 1999;Peters and Meyer). By subtracting a spec-

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From the acetone extract of roots of EUPHORBIA SIEBOLDIANA, three known diterpenes, ENT-atisane-3beta,16alpha,17-triol ( 1), ingenol ( 4), and helioscopinolide A ( 5) as well as two new diterpenes, 3beta- O-acetyl- ENT-atisane-16alpha,17-diol ( 2), and ingenol-20-palmitate ( 3) were isolated. Their structures were elucidated by spectroscopic methods and chemical transformations.

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