Recent study has reported that microRNA‐628‐5p (miR‐628‐5p) is involved in the development of epithelial ovarian cancer; however, the mechanisms of miR‐628‐5p in glioma remain unclear. In this study, we explored the potential biological roles of miR‐628‐5p in glioma. First, we found that miR‐628‐5p was decreased in the tissues and cells (U87 and T98) of glioma. Second, overexpressing miR‐628‐5p reduced the ability of glioma cells' proliferation and induced glioma cells' cycle arrest in G1. Then, we found that miR‐628‐5p directly bound to the 3′‐untranslated region of DDX59 and decreased the protein level of DDX59. The decrease of DDX59 was found to lead to the decrease of p‐AKT. Mechanistic studies revealed that restoring the expression of DDX59 alleviated miR‐628‐5p‐induced inhibition of proliferation of glioma. These findings suggest that the miR‐628‐5p/DDX59 axis has a key role in the development of glioma, and miR‐628‐5p might be a new therapeutic target against glioma.

Purpose Biomechanical comparison of wedge and biconcave deformity of different height restoration after augmentation of osteoporotic vertebral compression fractures was analyzed by three-dimensional finite element analysis (FEA). Methods Three-dimensional finite element model (FEM) of T11-L2 segment was constructed from CT scan of elderly osteoporosis patient. The von Mises stresses of vertebrae, intervertebral disc, facet joints, displacement, and range of motion (ROM) of wedge and biconcave deformity were compared at four different heights (Genant 0–3 grade) after T12 vertebral augmentation. Results In wedge deformity, the stress of T12 decreased as the vertebral height in neutral position, flexion, extension, and left axial rotation, whereas increased sharply in bending at Genant 0; L1 and L2 decreased in all positions excluding flexion of L2, and T11 increased in neutral position, flexion, extension, and right axial rotation at Genant 0. No significant changes in biconcave deformity. The stress of T11-T12, T12-L1, and L1-L2 intervertebral disc gradually increased or decreased under other positions in wedge fracture, whereas L1-L2 no significant change in biconcave fracture. The utmost overall facet joint stress is at Genant 3, whereas there is no significant change under the same position in biconcave fracture. The displacement and ROM of the wedge fracture had ups and downs, while a decline in all positions excluding extension in biconcave fracture. Conclusions The vertebral restoration height after augmentation to Genant 0 affects the von Mises stress, displacement, and ROM in wedge deformity, which may increase the risk of fracture, whereas restored or not in biconcave deformity.

Fusarium head blight (FHB) is one of the most detrimental wheat diseases. The accurate identification of FHB severity is significant to the sustainable management of FHB and the guarantee of food production and security. A total of 2752 images with five infection levels were collected to establish an FHB severity grading dataset (FHBSGD), and a novel lightweight GSEYOLOX-s was proposed to automatically recognize the severity of FHB. The simple, parameter-free attention module (SimAM) was fused into the CSPDarknet feature extraction network to obtain more representative disease features while avoiding additional parameters. Meanwhile, the ghost convolution of the model head (G-head) was designed to achieve lightweight and speed improvements. Furthermore, the efficient intersection over union (EIoU) loss was employed to accelerate the convergence speed and improve positioning precision. The results indicate that the GSEYOLOX-s model with only 8.06 MB parameters achieved a mean average precision (mAP) of 99.23% and a detection speed of 47 frames per second (FPS), which is the best performance compared with other lightweight models, such as EfficientDet, Mobilenet-YOLOV4, YOLOV7, YOLOX series. The proposed GSEYOLOX-s was deployed on mobile terminals to assist farmers in the real-time identification of the severity of FHB and facilitate the precise management of crop diseases.

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Background A new cannulated screw for the treatment of Pauwels type III femoral neck fracture (FNF) was developed and its biomechanical stability was analyzed by finite element analysis and mechanical tests to provide a new approach for the clinical treatment of Pauwels type III FNF. Methods A Pauwels III femoral neck fracture model with an angle of 70° was constructed using medical engineering software such as Mimics, Geomagic, and Hypermesh. Two parallel inverted triangular cannulated screw finite element models were established to simulate surgical fixation: three conventional cannulated screw models(CCS), and two upper cannulated screw + one new cannulated screw models(NCS). The biomechanical properties of the 2 fixation methods were compared and analyzed under the same loading and constraint conditions. An unstable femoral neck fracture model was made using a composite femur (Sawbone) at a Pauwels 70° angle. Reduction and fixation models of Pauwels III FNF were made according to CCS and NCS fixation methods. and the compression displacement of the femur was tested, then the load-displacement curve was used to calculate the stiffness and compare the advantages and disadvantages of the two internal fixation methods. Results In the computer-simulated reconstruction of the inverted triangular cannulated screw fixation model for Pauwels type III FNFs, the NCS model outperformed the CCS model in terms of strain and stress distribution. And the peak stress of the 2 groups appeared in the middle section of the screw closest to the femoral spur, close to the fracture line. In the experimental mechanical model of Pauwels type III FNF, under the same loading condition, the NCS group has smaller distance of femoral deformation and fracture end displacement than the CCS group, and from the perspective of stiffness, the former group had greater stiffness and smaller compression distance under the same vertical load. Conclusions The new cannulated screws provide good biomechanical stability for the treatment of Pauwels type III FNFs, which provides a new choice for the treatment of young patients with vertical fracture of femoral neck.