Glioblastoma multiforme (GBM), the most aggressive and lethal primary brain tumor, is characterized by very low life expectancy. Understanding the genomic and proteogenomic characteristics of GBM is essential for devising better therapeutic approaches.Here, we performed proteomic profiling of 8 GBM and paired normal brain tissues. In parallel, comprehensive integrative genomic analysis of GBM was performed in silico using mRNA microarray and sequencing data. Two whole transcript expression profiling cohorts were used - a set of 3 normal brain tissues and 22 glioma tissue samples and a cohort of 5 normal brain tissues and 49 glioma tissue samples. A validation cohort included 529 GBM patients from The Cancer Genome Atlas datasets. We identified 36 molecules commonly changed at the level of the gene and protein, including up-regulated TGFBI and NES and down-regulated SNCA and HSPA12A. Single amino acid variant analysis identified 200 proteins with high mutation rates in GBM samples. We further identified 14 differentially expressed genes with high-level protein modification, among which NES and TNC showed differential expression at the protein level. Moreover, higher expression of NES and TNC mRNAs correlated with shorter overall survival, suggesting that these genes constitute potential biomarkers for GBM.

Chitosan coated bentonite (CCB) was prepared by microwave irridiation, original bentonite as raw materials and chitosan as modifier. The adsorption performance in acid scarlet dye solution was discussed. Firstly the optimum adsorption conditions: concentration of chitosan of 35 g/L, bentonite quality and chitosan solution volume rate of 0.7(g/L), microwave time of 17 min, infilltration time of 50 min, deacetylation degree of chitosan of 85%, soil particle size of 15 mm, pH of 5, dosage of CCB of 20 g/g, stirring speed of 300 r/min, centrifugal time of 15 min, and stirring time of 35 min, were investigated by single factor experiment. And the maximum adsorption rate reached to 96.2%. Subsquently, all the effect tendencies of the physicochemical parameters of the preparation and adsorption were studied on the optium experimental condition, which would provide reference data for the subsequent pilot test. The results suggested that CCB was a promising sorbent to remove organic contaminants and might be utilized to purify water contaminated by organic compounds.

A new type of adsorbent which prepared with waste paper as raw material was applied in the treatment of dye wastewater, and studied the processing conditions on treatment effect. Batch adsorption study carried out as a function of the dosage, the stirring time, the stirring speed and the pH. The adsorption rate of newspaper adsorbent to basic blue dye simulated wastewater and alkaline yellow wastewater with an initial concentration of 80mg·L-1 can reach 98.56% and 92.23% at 4g·L-1 of the dosage of paper adsorbent, 15min of the stirring time and 150r·min-1of the stirring speed. The maximum adsorption were 233.1g·L-1 and 289.4g·L-1 respectively. The isothermal adsorption experiment was also studied and concluded that the adsorption equilibrium data fitted with the Langmuir isotherm. The characterization experiment showed that the modified paper adsorbent had stronger absorbability.