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This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.

Highlights BioAider is an efficient tool for high-throughput analysis of viral genomes. BioAider monitors viral variation that facilitates epidemic control of COVID-19. 14 substitution hotspots in SARS-CoV-2 genome indicates viral polymorphism. NSP13-Y541C was found to be a crucial substitution key viral replication. The unique SSRX repeats on N protein suggests the animal origin of SARS-CoV-2.

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