In this study, an efficient polysaccharide, named CAL-n (Crinum asiaticum L.-n) was isolated and purified from Crinum asiaticum L for the first time, Mw(molecular weight) of 730,000 Da. CAL-n comprised Rha(rhamnose), Sor(sorbose), Gal(galactose) and Glu(glucosein) the molar ratio of 1:61.6:1.66:4.74. The chemical structure of CAL-n was studied by Infrared spectrum and GC–MS(Gas Chromatography–Mass Spectrometer) analysis. Experimental results reflected, that the backbone of CAL-n comprised (1 → 2), (1 → 6), (1 → 3) beta-pyran glycoside bond, without (1 → 4) beta-pyran glycoside bond. In addition, an MTT assay indicated that the growth of HepG2 cells was affected by CAL-n, with a concentration dependant ration. The results indicated that CAL-n should by exploration as anti-tumor activities in vivo.

Background Recent scientific research has enabled the identification of macrophages related-genes (MaRG), which play a key role in the control of the immune microenvironment in many human cancers. However, the functional role of MaRGs in human tumors is ill-defined. Herein, we aimed at bioinformatically exploring the molecular signatures of MaRGs in colorectal cancer. Methods A list of MaRGs was generated and their differential expression was analyzed across multiple datasets downloaded from the publicly available functional genomics database Gene Expression Omnibus. The weighted gene co-expression network analysis (WGCNA) was also applied to identify the partner genes of these MaRGs in colorectal cancer. Results After integration of the results from analyses of different datasets, we found that 29 differentially expressed MaRGs (DE-MaRGs) could be considered as CRC-related genes as obtained from the WGCNA analysis. These genes were functionally involved in positive regulation of DNA biosynthetic process and glutathione metabolism. Protein–protein interaction network analysis indicated that PDIA6, PSMA1, PRC1, RRM2, HSP90AB1, CDK4, MCM7, RFC4, and CCT5 were the hub MaRGs. The LASSO approach was used for validating the 29 MaRGs in TCGA-COAD and TCGA-READ data and the results showed that ten among the 29 genes could be considered as MaRGs significantly involved in CRC. The maftools analysis showed that MaRGs were mutated at varying degrees. The nomogram analysis indicated the correlation of these MaRGs with diverse clinical features of CRC patients. Conclusions Conclusively, the present disclosed a signature of MaRGs as potential key regulators involved in CRC pathogenesis and progression. These findings contribute not only to the understanding of the molecular mechanism of CRC pathogenesis but also to the development of adequate immunotherapies for CRC patients.

Andrographis paniculata (Burm. f.) Nees (APN) is a medicinal plant widely used in Asian countries. APN is used for its anti-inflammatory and antiviral properties. Previous studies did not clarify its pathway to treat hepatitis under the next generation of natural antibiotics. No definite trend has found expression in the multi-component Chinese medicine (MCCM). Besides, the research and development of a particular component in APN can not cover the most pharmacological effects of APN treating hepatitis. Based on the current information biology data, this review found that the intersection number of APN and hepatitis were 1,016 from 3,922 APN PPI targets and 1,818 hepatitis targets, respectively, according to current data. The filtered prediction outcome suggests that the MCCM of APN treating hepatitis were wogonin, oroxylin A, and andrographolide, which accounted for more than 95% of the compound contribution index of APN treating hepatitis. The predicated primary related pathways were the Notch, thyroid hormone, hepatitis B, and prolactin signalling pathways. This review serves to summarize the current research on APN to indicate a specific research direction to determine the pharmacological mechanism of APN and the interaction between drug targets and disease targets, laying a foundation for the subsequent development of its MCCM, new drugs, and auxiliary drugs.