Endometrial epithelial cells carry distinct cancer-associated alterations that may be more susceptible to endometriosis. Mouse models have shown that overexpression of SIRT1 associated with oncogene activation contributes to the pathogenesis of endometriosis, but the underlying reason remains elusive. Here, we used integrated systems biology analysis and found that enrichment of endometrial stromal fibroblasts in endometriosis and their cellular abundance correlated negatively with epithelial cells in clinical specimens. Furthermore, endometrial epithelial cells were characterized by significant overexpression of SIRT1, which is involved in triggering the EMT switch by escaping damage or oncogene-induced induced senescence in clinical specimens and in vitro human cell line models. This observation supports that genetic and epigenetic incident favors endometrial epithelia cells escape from senescence and fuel EMT process in endometriosis, what could be overcome by downregulation of SIRT1.

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Rural tourism is an important driving force for the transformation of rural society in the process of urbanization. However, besides promoting rural economy, it also interrupts the development of the rural living, human, manage and economic multi-dimensional environments. Space reconstruction in rural settlements is an important means to optimize multi-dimensional environments and revitalize rural tourism. Focusing on two types of rural settlements, namely the “relocation village” and the “sightseeing and settlement symbiosis village”, this study has revealed the affecting mechanism of space reconstruction in rural tourist locations on the local multi-dimensional environment by establishing a multi-dimensional environment evaluation system through an analytic, hierarchical process. Subsequently, based on the theoretical perspective of the reproduction of the space and the paradigm of the three-fold model, a comprehensive optimization path of rural multi-dimensional environments has been proposed. The results show that in the space reconstruction process driven by tourism: (1) the multi-dimensional environments of the “sightseeing and settlement symbiosis village” generally develop better than the “relocation village”; (2) the ecological environment is the direct benefit while the management environment and economic environment are the bottlenecks; (3) the “sightseeing and settlement symbiosis village” is more appealing to tourists who are interested in rural tourism since it keeps more rustic characteristics than the “relocation village”; (4) in order to optimize the multi-dimensional environments, “relocation village” should pay more attention to the space needs of residents, improve space utilization rates, and increase the residents’ sense of belonging; (5) “sightseeing and settlement symbiosis village” needs to resolve the contradiction between the tourism and living demands, improve the reproduction process driven by diversified entities, and reconstruct the neighborhood network under rural tourism.

Our previous work reported that galaxamide, a cyclopeptide extracted from the seaweed Galaxaura filamentosa, showed antiproliferative activity against HeLa cells by MTT assay. In this study, the growth‐inhibitory effects of galaxamide in HeLa cells and xenograft mouse models were investigated. It was found galaxamide significantly inhibited cell growth, colony formation, migration, and invasion and induced cell apoptosis by inhibiting the Wnt signaling pathway in HeLa cells. RNA sequencing revealed that galaxamide regulated stemness by Wnt6 signaling pathway in HeLa cells. By analyzing The Cancer Genome Atlas database, Wnt6 was found to be negatively/positively correlated with stemness‐ and apoptosis‐related genes in human cervical cancer. Cancer stem‐like cells (CSCs) isolated and enriched from HeLa cells demonstrated elevated Wnt6 and β‐catenin genes compared with nonstem HeLa cells. After galaxamide treatment, CSCs showed abrogation of sphere‐forming ability, along with inhibition of stemness‐related and Wnt pathway genes. Galaxamide treatment was also accompanied by the induction of apoptosis in HeLa cells, which was consistent with the results in BALB/c nude mice. Our results provide evidence that suppression of stemness by downregulating the Wnt signaling pathway is the molecular mechanism by which galaxamide effectively inhibits cell growth and induces apoptosis in cervical cancer cells.

Modernized urban organization relies more on the foundation of ecological infrastructure (EI) with the planning objectives based more on synthetical benefits of economy, society and ecological environment. The effect of multiple factors ought to be taken into consideration in the urban scenic spot planning and and the process of formulating the related plan faces a challenge of how to organically bound environmental factors to tour function of urban scenic spot. This thesis, taking YunLong Lake, a scenic spot in XuZhou, JiangSu province as an example, studies how to ameliorate the planning of urban scenic spot combined with the ecological chain and reversed-planning theory. The result shows that utilizing the theory flexibly when analyzing the urban scenic spot planning design can ensure the existing ecological connection between each system and maintain the ecological stability of urban scenic spot. Thus, with the basis of ecological chain and reversed-planning theory, this paper puts forward a new planning concept and work flow, in order to provide new ideas for modernized urban scenic spot planning.