Abstract:The fat-1 transgenic mice were used as models, to investigate the effects of n-3PUFAs on body weight, the expression of inflammation and autophagy in mice, and to explore its mechanism. The mice were divided into two groups: fat-1 transgenic mice and wild-type mice. The body weight and body length were measured and the index of body weight/body length was calculated regularly every week for 8 weeks. Cardiac blood was collected for determination of TG, CT, HDL-C, LDL-C and BG. Frozen sections of liver were stained by Oil Red O to observe the lipid droplets. The expression of autophagy proteins P62, LC3 and ATG7 in the hypothalamus were detected by western blot, and the relative quantitative analysis was performed. Real-time quantitative PCR was used to quantify the mRNA expression of TNF-α, IL-6, IL-1β, IFN-γ, MCP-1, TLR-4 and adiponectin in epididymal adipose tissue. The body weight/body length of fat-1 transgenic mice was significantly lower than that of wild-type mice(P<0.05), the levels of TG, CT, HDL-C, LDL-C and BG in the serum of fat-1 transgenic mice were significantly lower than those in wild-type mice(P<0.05). Lipid droplets in the liver of fat-1 transgenic mice were significantly less. The expression of P62 in fat-1 transgenic mice was significantly down-regulated (P <0.05), while the expression of ATG7 was significantly increased (P <0.05), and the ratio of LC3 Ⅱ / Ⅰ was significantly increased(P<0.05). The results of real-time quantitative PCR showed that the mRNA relative expression of TNF-α, IL-6, IL-1β, IFN-γ, MCP-1 and TLR-4 in epididymal fat tissue of fat-1 transgenic mice was significantly decreased, and the expression of adiponectin was increased (P < 0.05). n-3PUFAs reduce the body weight to prevent obesity may by up-regulation of hypothalamic autophagy, and down-regulation of inflammation in peripheral fat.

Objective: Exploring the expression of cell-free RNA in urine of normal patients and lung cancer patients, analyze its clinical value in the dignosis of lung cancer. Methods: The urine samples were collected from lung cancer patients and healthy people. Extracted the total cf-RNA from all samples. The contents of total cf-RNA in two groups were detected, and the expressions of LUNX mRNA in two groups were measured by quantitative real-time PCR. Receiver-operating characteristics (ROC) curve was established to evaluate the diagnostic value of urine cf-RNA for the differentiation between lung cancer patients and the control group. Results: Compare to the normal group (50.50±4.527ng), the urinary cf-RNA content in patients (133.8±8.615ng) was significantly increased, p<0.05. The expression of urinary lunx mRNA level from patients was raised, p<0.05. ROC curve analysis showed that, in the diagnosis, AUC value of lunx mRNA was 0.83(95% CI, 0.747-0.906), p<0.05. Conclusion: The expression of urine cf-RNA and Lunx mRNA was elevated, and can be used as a potential molecular diagnostic tool for lung cancer.