An azido pyridoxal derivative (PLAA) was developed as a protein azidolation reagent, with selectivity towards N-terminal glycine. Successful PLAA modification was achieved with small peptide, natural proteins and lab-expressed protein...

The wide spread of metallo-β-lactamase (MBL)-expressing bacteria has greatly threatened human health, and there is an urgent need for inhibitors against MBLs. Herein, we present a cephalosporin-tripodalamine conjugate (DPASC) as a potent MBL inhibitor with a block-release design.

Since 2003, China has made extraordinary achievements in emergency management, and successfully dealt with a large number of unexpected events, especially meteorological disasters. At the same time, a complete system of emergency laws, regulations and plans are gradually established. Through reviewing those official documents, this paper finds out four important principles that typhoon disaster emergency responses follows: warning level based, disaster consequences and types oriented, unified leadership and departmental response, decentralized response and localized management.

<p>There has been a lack of academic research focusing on the generational differences in leadership preferences in the Chinese context, particularly in terms of the Millennial generation. In what way, if at all, do the leadership preferences of Chinese Millennials differ from those of Chinese non-Millennials? Such was the guiding question of the study I present in this thesis. The purpose of this study was to explore and compare the leadership preferences of Chinese Millennials and non-Millennials, so as to allow academics and organizations to better understand the leadership preferences of Chinese Millennials, and recognize the differences/similarities in leadership preferences between the employees of different generations in China. A total of 460 participants participated in this study (230 are Millennials, 230 non-Millennials), all of whom were Chinese living in mainland China, and full-time workers with at least one year of work experience. The study used a questionnaire to collect data regarding participants’ leadership preferences, which was then analyzed in three steps (i.e., a primary analysis and two follow-up analyses). The results show that, overall, Chinese Millennials have similar leadership preferences to non-Millennials. I draw on relevant literature to theorize about the social and psychological mechanisms that might underpin my findings. Potential explanations include traditional Chinese culture being consolidated and inherited; people having similar fundamental needs (regardless of generational membership), and therefore similar leadership preferences; and the impact of intergenerational interactions and a shared organizational environment. By increasing understanding of the research evidence that exists about leadership preferences across generations, leadership challenges in the multi-generation workforce can be overcome. A better understanding of Millennials’ leadership preferences, as well as differences and similarities in these preferences among different generations, also enables organizations and leaders to better lead the growing number of Millennial employees in China.</p>

Background: Hepatocellular carcinoma is one of the most aggressive gastrointestinal tumor, with a high recurrence and mortality rate. Novel immunotherapy targeting the tumor microenvironment are innovative and promising therapeutic approaches for some tumor, including hepatocellular carcinoma. However, patr of patients with HCC remain a diminished response to immunotherapy on account of the insufficient understanding on the tumor microenvironment. Methods: The correlation between infiltrated immune cells and hepatocellular carcinoma patients prognosis were confirmed via MCP-counter algorithms. The single-cell RNA sequencing dataset (GSE146115) was performed to identify T cells marker genes in hepatocellular carcinoma. GSE10141, GSE14520 dataset and TCGA-liver hepatocellular carcinoma were used to construct and validate the T cell marker genes signature (TCMS).Results: The survival analysis revealed the correlation between infiltrating T cells and the overall survival of patients with hepatocellular carcinoma. Four T cell marker genes included in the TCMS model were: HELZ, GZMA, SLC2A2, JAK3. The TCMS risk score could stratify patients with hepatocellular carcinoma into high- and low-risk score subgroups. TCMS risk score remained a influential feature of overall survival and one of the independent prognostic risk factors in multivariate analysis in TCGC validation cohort. Besides, correlation analysis indicated increased expression of HELZ, GZMA and JAK3 were significantly correlated with high degree of CD8+ T cell, CD4+ T cell infiltration.Conclusion: We successfully constructed the T cell marker genes signature with powerful predictive function and provided a new understanding of T cell infiltration in tumor microenvironment which might offer practices instructions for HCC immunotherapy.