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Mucins, produced by epithelial tissues in most metazoans, have served as reliable molecular biomarkers for cancer diagnosis and prognosis. In this work, a competitive electrochemical aptasensor is reported for the determination of mucin 1 protein by p-aminophenol redox cycling. Specifically, the conjugates produced between biotinylated mucin 1 and streptavidinalkaline phosphatase were captured by an anti-mucin 1 aptamer-modified electrode, which induced the production of electrochemically active p-aminophenol from the p-aminophenyl phosphate substrate. The resulting p-aminophenol was cycled by tris(2-carboxyethyl) phosphine after its oxidization on the electrode, thus enabling an increase in the anodic current. Because the mucin 1 competed with the conjugates to bind the anchored aptamer, the signal decreased with an increase in protein concentration between 0.5 and 6 nM. As a result, a detection limit of 0.1 nM was achieved. To demonstrate the viability of the method for real samples, the effects of glucose, blood serum, and other proteins (e.g., thrombin, human immunoglobulin G, and Zn 7 -metallothionein) were investigated.

An LCC/SP self-tuning wireless charging system is proposed herein for use in a wireless charging test bench. With different dislocations in addition to changes in the coil self-inductance and mutual inductance caused by different secondary magnetic shielding materials, the system can ensure that the high power factor of the primary side remains unchanged without changing the circuit topology. Based on this normalized detuning LCC/SP circuit model, a switch-controlled capacitor (SCC) self-tuning method based on PI control is proposed. The control scheme employs only two MOSFETs and capacitors, without WIFI communication or parameter identification. A 2 kW experimental device was set up in the laboratory, and experimental verification was carried out with large-scale dislocations and different secondary magnetic shielding materials. The experimental results confirm that the system can maintain a high power factor (>0.9) under a system mutual inductance variation of 47.7% and secondary coil self-inductance variation of 12%, and that it can be applied in electric vehicle wireless chargers or high-power wireless charger test benches.

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