* Autor a quien debe ser dirigida la correspondenciaRecibido Ene. 5, 2015; Aceptado Mar. 12, 2015; Versión final Abr. 25, 2015, Publicado Oct. 2015 Resumen En la presente investigación se elaboraron biopelículas activas a base de alginato, almidón de maíz y extracto acuoso de toronjil (Melissa officinalis L), evaluando su incidencia en la vida útil microbiológica del camarón blanco del pacifico (Penaeus vannamei). Para la estimación de la vida útil se realizaron cinéticas de crecimiento de microorganismos indicadores (aerobios mesófilos) modeladas matemáticamente utilizando la ecuación de Baranyi y Robert utilizando el programa DMFIT para obtener los parámetros cinéticos de crecimiento microbiano a diferentes temperaturas. Finalmente la ecuación de MonodHinshelwood fue utilizada para estimar la vida útil de los camarones. Los resultados indicaron que es posible utilizar biopelículas a base de alginato, almidón de maíz y como sustancia antimicrobiana extracto acuoso de toronjil para la conservación de camarones (Penaeus vannamei), debido a que se obtuvieron incrementos del 33% aproximadamente en la vida útil de camarones (Penaeus vannamei) recubiertos con las biopelículas. Palabras clave: alginato, almidón de maíz, biopelículas activas, vida útil Obtaining Active Binary Biofilm and its Effect on Microbiological Shelf Life of White Shrimp (Penaeus Vannamei) AbstractIn this research active biofilms based on alginate, cornstarch and water extract of lemon balm (Melissa officinalis L) were developed, assessing their impact on the microbiological shelf life of Pacific white shrimp (Penaeus vannamei). Microbial growth kinetics using as indicators microorganism aerobic mesophilic bacterial modeled mathematically through Baranyi y Robert equation by the program DMFIT were used for determinate the microbial growth parameters at different temperatures. Finally Hinshelwood -Monod equation was used to estimate the shelf life of the shrimp. The results indicated that is possible obtain binary biofilms containing aqueous extract of lemon balm for the shrimp conservation, because increments of 6 days were obtained in the shelf life of shrimp coated with biofilms
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