In the inner plexiform layer (IPL) of the mouse retina, ~70 neuronal subtypes organize their neurites into an intricate laminar structure that underlies visual processing. To find recognition proteins involved in lamination, we utilized microarray data from 13 subtypes to identify differentially-expressed extracellular proteins and performed a high-throughput biochemical screen. We identified ~50 previously-unknown receptor-ligand pairs, including new interactions among members of the FLRT and Unc5 families. These proteins show laminar-restricted IPL localization and induce attraction and/or repulsion of retinal neurites in culture, placing them in an ideal position to mediate laminar targeting. Consistent with a repulsive role in arbor lamination, we observed complementary expression patterns for one interaction pair, FLRT2-Unc5C, in vivo. Starburst amacrine cells and their synaptic partners, ON-OFF direction-selective ganglion cells, express FLRT2 and are repelled by Unc5C. These data suggest a single molecular mechanism may have been co-opted by synaptic partners to ensure joint laminar restriction.DOI: http://dx.doi.org/10.7554/eLife.08149.001

A content analysis was conducted to examine the portrayal of beauty types and occupational roles of female figures in magazine advertisements. A systematic random sample of 215 advertisements from a popular lifestyle magazine in Hong Kong was analyzed. Results provide evidence of gender role stereotypes. Two thirds of the advertisements adopted classic/feminine beauty type. The other two common beauty types were sex kitten and casual. Over-representation of Caucasian models was found as one-third of the female figures were Caucasian and other minorities. Caucasian models were more likely to be portrayed in trendy beauty type. Over half of the advertisements portrayed females in decorative roles and thirty percent portrayed females as celebrities. Recreational roles and professional roles were featured less frequently. Advertisements for products target females were more gender stereotyped than advertisements for products target males and females. The lack of variety in beauty types and occupational roles among female figures were discussed.

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