Background: Cooperation between families of individuals with mental illnesses and mental health professionals is very important for the quality of mental health care, and there are many barriers to the establishment of cooperation between mental health professionals and families. It is highly important to identify/define barriers to cooperation between families and health professionals from a cultural perspective. Aim: The present study was aimed at identifying barriers to cooperation between mental health professionals and families from the perspectives of mental health professionals and family caregivers in Turkey. Method: In the sample of this descriptive qualitative study, 12 family caregivers and 11 health professionals were included. Results: The results of the study indicated seven themes regarding the perceptions of family caregivers and mental health professionals. The themes related to perceptions of family caregivers about barriers are as follows: “learning the process by living,” the perception of “my patient comes first,” and the perception of “being neglected.” The themes related to perceptions of mental health professionals about barriers are as follows: “lack of collaboration within the team,” “family itself as a barrier,” and “lack of education about working with families.” The common theme mentioned by both groups included the “patient-oriented service understanding.” Conclusions: The results obtained from this study are believed to be a guide for planning and implementing interventions to eliminate the barriers defined from the perspectives of both mental health professionals and family caregivers. Both mental health professionals and family caregivers need psychosocial interventions for strengthening “family cooperation.”