Potassium is important for plant growth and crop yield. However, the effects of potassium (K+) deficiency on silage maize biomass yield and how maize shoot feedback mechanisms of K+ deficiency regulate whole plant growth remains largely unknown. Here, the study aims to explore the maize growth, transcriptional and metabolic responses of shoots to long-term potassium deficiency. Under the K+ insufficiency condition, the biomass yield of silage maize decreased. The transcriptome data showed that there were 922 and 1,107 differential expression genes in DH605 and Z58, respectively. In the two varieties, 390 differently expressed overlapping genes were similarly regulated. These genes were considered the fundamental responses to K+ deficiency in maize shoots. Many stress-induced genes are involved in transport, primary and secondary metabolism, regulation, and other processes, which are involved in K+ acquisition and homeostasis. Metabolic profiles indicated that most amino acids, phenolic acids, organic acids, and alkaloids were accumulated in shoots under K+ deficiency conditions and part of the sugars and sugar alcohols also increased. It revealed that putrescine and putrescine derivatives were specifically accumulated under the K+ deficiency condition, which may play a role in the feedback regulation of shoot growth. These results confirmed the importance of K+ on silage maize production and provided a deeper insight into the responses to K+ deficiency in maize shoots.

AimsPotassium is important for plant growth and crop yield. However, the effects of potassium (K+) deficiency on silage maize biomass yield and how maize shoot feedback mechanisms of K+ deficiency regulating whole plant growth remains largely unknown. Here, the study aims to explore the maize growth and transcriptional and metabolic responses of shoots to long-term potassium deficiency.MethodsThe growth of silage maize and its biomass were analyzed with K+ treatment in field and hydroponic experiments. Furthermore, transcriptional and metabolic profiles of shoots were investigated for their effects on maize development under K+ deficiency condition. ResultsUnder K+ insufficiency condition, the biomass yield of silage maize decreased by 14%-17% in two-year field trials. The transcriptome data showed that there were 390 differently expressed genes overlapping and similarly regulated in the two varieties and they were considered as the fundamental responses to K+ deficiency in maize shoots, with many stress-induced genes involved in transport, primary and secondary metabolism, regulation, and other processes involved in K+ acquisition and homeostasis. Metabolic profiles indicated that most amino acids, phenolic acids, organic acids, and alkaloids were accumulated in shoots under K+ deficiency condition and part of the sugars and sugar alcohols also increased. ConclusionOur results suggested putrescine and putrescine derivatives were specifically accumulated under K+ deficiency condition, which may play a role in feedback regulation of shoot growth. These results confirmed the importance of K+ on silage maize production and provided a deeper insight into the responses to K+ deficiency in maize shoots.