An autopsy case of a 38‐year‐old man demonstrating numerous peculiar membrane‐structures composed of compound lipid in the meta‐physis and marrow of long bones and various organs, where physiological hyperplasia ex vacuo of adipose tissue has occurred remarkably, is reported. The membranous structure was not observed in the brain while sudanophilic leucodystrophy was prominent. The cause of this case is assumed to be an abnormality in the process of differentiation and proliferation of mesenchymal cell to fat cell in adult age, in which the lipid is combined with mucopolysaccharide in the ground substance of connective tissue, resulting in formation of peculiar membranous structure. ACTA PATH. JAP. 23: 539–558, 1973.

A dual staining method has been developed to identify two types of mucous secreting cells in the gastric mucosa of human and rat in one and the same tissue section. Sections were stained first using the galactose oxidase-cold thionin Schiff (GOCTS) procedure and then with paradoxical Concanavalin A staining (PCS). Surface mucous cell mucin stained blue with GOCTS, whereas gland mucous cell mucin stained brown with PCS. This method enabled us to differentiate these two types of mucins not only in gastric epithelial cell cytoplasm but also in the extracellular space. Sugar residues detected by GOCTS were explored by employing four species of lectins, which were peanut and Allomyrina dichotoma agglutinins for beta-galactose and Vicia villosa and Wistaria floribunda agglutinins for beta-N-acetylgalactosamine. The effect of oxidation with galactose oxidase was also examined on the affinities of reactive sites for these lectins. The results indicated that, in the human stomach, the sugar residues responsible for this reactivity were most likely beta-N-acetylgalactosamine and beta-galactose in specimens lacking secretion of blood group determinants and beta-N-acetylgalactosamine in those showing the secretion. In the rat stomach, on the other hand, sugar residues responsible for GOCTS were not elucidated by these lectins.

No abstract

A case of bilateral gonadoblastoma in a phenotypic female with 45, X/46, XY gonadal dysgenesis is presented. Hormonal investigations revealed that serum testosterone, estradiol and Β-human chorionic gonadotropin decreased following excision of the tumors, but follicle-stimulating hormone and luteinizing homrone levels increased further. Immunohistochemical staining for testosterone and estradiol was positive in both Leydig and lutein-like cells in the tumor. It is suggested that gonadoblastoma is capable of producing testosterone and estradiol, and Leydig or lutein-like cells may be the actual source of these steroid hormones.

Immunohistochemical staining was performed on normal, endometriotic, neoplastic endometrial tissues and various epithelial tissues of other organs using a monoclonal antibody, 5D4, which was raised originally against skeletal and corneal keratan sulfate. The results showed that this antibody exhibited strong and consistent reactivity to glandular epithelia found in normal and endometriotic tissues. In endometrial carcinomas, moderate reactivity was noted in well- to moderately differentiated adenocarcinoma. Other epithelial tissues examined showed almost no or faint reactivities. These results suggested that 5D4 would be a useful tool for identifying the endometrial glandular epithelia in histochemical studies.