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Through bioassay and biolegical investigations on the alarm pheromone of five species of acarid mites, Alettroglyphzts ovattes, CZirpoglt!phus lactis, Lardbglyphus konoi and Dermatophagoides forinae. following results were obtained. Citral and nery1 formate were more er less active as an alarm pheromone and not strictly species-specific within five species. Along with T. Putrescentiae, two more acarid mites, C lactis and L. konoi, were concluded to possess the alarm pheromone and citral was identified as their pheromone. Citral was emitted to the environment and the quantity of this discharge was 10 to 50 times larger when mites were disturbed than when undisturbed. Furthermore the composition of citral differed significantly between the disturbed (74-86% of neral) and undisturbed conditions (54-66% of neral), that is, the percentage of neral increased when disturbed. The original composition of extracted citral under 1-min soaking consisted of more than 90% of neral in the three species except L. konoi (75% of neral). Therefore the initial composition of citral when discharged from glands is supposed to consist of more than 90% of neral in three species, L. konoi, A. owatus and D. fLirinae. Secretory gland of citral was identified as the latero-abdominal gland or the oil gland in all species of mites.

Pheromone study on acarid mites II Presence of the alarm pheromone in the mold mite,

From an acarid mite, Lardoglyphus konoi (Sasa et Asanuma) (Acarina: Acaridae), with its culture medium,an aggregation pheromonewas isolated. The structure of the pheromonewas determined as 1,3,5,7-tetramethyldecyl formate and identified by 1 1 steps of organic synthesis. The absolute configuration of Q was assigned as R. The compound has been assigned the trivial name lardolure. The synthesized diastereomeric mixture of racemates showed one-tenth of the activity of the natural pheromone against the test mite, Carpoglyphus lactis.