The reliability of the squat jump force-velocity and loadvelocity profiles. J Strength Cond Res 36(11): 3000-3007, 2022-The purpose of this study was to investigate the between-session reliability of the squat jump force-velocity (FV) and load-velocity (LV) profiles. Eighteen subjects (age 5 28.1 6 4.8 years; height 5 1.7 6 9.7; body mass 5 74.7 6 12.8) who could back squat .1.5 times body mass participated in this study. Each subject completed a familiarization session, followed by 2 experimental sessions each separated by 72 hours. Subjects performed a series of squat jumps on a force plate against external loads between 0 and 100% of their body mass in a quasi-randomized block order. Intraclass correlation coefficient (ICC) and coefficient of variation (CV) were used to examine the between-session reliability. Peak velocity (PV) and mean velocity (MV) at each load were highly reliable (ICC .0.80, CV% ,7.41, SEM ,0.13 m•s 21 , smallest detectable difference [SDD] ,0.31 m•s 21 , ES ,0.21). Force-velocity profiles created with peak force and relative peak force resulted in poor to excellent reliability (ICC 5 0.34-0.92, CV% 5 11.9-26.3). When mean and relative mean forces were used to create FV profiles, there was poor to good reliability (ICC 5 0.03-0.85, CV% 5 18.1-39.4). When the LV profile was calculated with PV (ICC 5 0.60-0.90, CV% 5 7.9-16.9) or MV (ICC 5 0.49-0.91, CV% 5 11.1-23.4), there was poor to excellent reliability. There was no time effect found between sessions for both FV and LV profiles. The squat jump FV and LV profiles established with a force plate are not reliable. Therefore, these profiles are not recommended to be used to inform programming decisions.

A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC(50) values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.

Guppy, SN, Brady, CJ, Kotani, Y, Stone, MH, Medic, N, and Haff, GG. Effect of altering body posture and barbell position on the within-session reliability and magnitude of force-time curve characteristics in the isometric midthigh pull. J Strength Cond Res 33(12): 3252–3262, 2019—A large degree of variation in the position used during isometric midthigh pull (IMTP) testing and conflicting results of the effects of these changes can be found in the literature. This study investigated the effect of altering body posture and barbell position on the reliability and magnitude of force-time characteristics generated during the IMTP. Seventeen strength-power athletes (n = 11 males, height: 177.5 ± 7.0 cm, body mass: 90.0 ± 14.1 kg, age: 30.6 ± 10.4 years; n = 6 females, height: 165.8 ± 11.4 cm; body mass: 66.4 ± 13.9 kg, age: 30.8 ± 8.7 years) with greater than 6 months of training experience in the clean (1 repetition maximum: 118.5 ± 20.6 kg, 77.5 ± 10.4 kg) volunteered to undertake the experimental protocol. Subjects performed the IMTP using 4 combinations of hip and knee angles, and 2 different barbell positions. The first barbell position corresponded to the second pull of the clean, while the second rested at the midpoint between the iliac crest and the patella. Peak force (PF), time-specific force (F50, F90, F150, F200, and F250), peak rate of force development (pRFD), and impulse (IMP) time bands were reliable in all 4 testing positions examined. Statistically greater PF, F50, F90, F150, F200, F250, pRFD, and IMP0–50, IMP0–90, IMP0–150, and IMP0–200 were generated in a testing position corresponding to the second pull of the clean when compared with a bent over torso angle, regardless of the barbell position used. Moderate to large effect sizes favoring a testing position corresponding to the second pull were also found. Overall, when performing the IMTP, an upright torso and a barbell position that matches the second pull of the clean should be used.

It is important to select an appropriate surrogate matrix for preparing calibration standards and quality control samples while quantitatively assaying for endogenous substances, because a blank matrix that does not contain the endogenous substance cannot be derived from the species from which the target study samples are collected. This is because the assay results might be affected, depending on the characteristics of the analyte in the surrogate matrix. Our discussion group that participated in the Japan Bioanalysis Forum discussed the recommended selection strategies, focusing on large and small molecules in ligand binding assays and LC–MS, respectively. We established an efficient selection strategy for a surrogate matrix, with simple compositions as the first candidates stated in this article.

Seventeen strength and power athletes (n = 11 males, 6 females; height: 177.5 ± 7.0 cm, 165.8 ± 11.4 cm; body mass: 90.0 ± 14.1 kg, 66.4 ± 13.9 kg; age: 30.6 ± 10.4 years, 30.8 ± 8.7 years), who regularly performed weightlifting movements during their resistance training programs, were recruited to examine the effect of altering body posture and barbell position on the between-session reliability of force-time characteristics generated in the isometric mid-thigh pull (IMTP). After participants were familiarised with the testing protocol, they undertook two testing sessions which were separated by seven days. In each session, the participants performed three maximal IMTP trials in each of the four testing positions examined, with the order of testing randomized. In each position, no significant differences were found between sessions for all force-time characteristics (p = >0.05). Peak force (PF), time-specific force (F50, F90, F150, F200, F250) and IMP time-bands (0–50, 0–90, 0–150, 0–200, 0–250 ms) were reliable across each of the four testing positions (ICC ≥ 0.7, CV ≤ 15%). Time to peak force, peak RFD, RFD time-bands (0–50, 0–90, 0–150, 0–200, 0–250 ms) and peak IMP were unreliable regardless of the testing position used (ICC = <0.7, CV = >15%). Overall, the use of body postures and barbell positions during the IMTP that do not correspond to the second pull of the clean have no adverse effect on the reliability of the force-time characteristics generated.