Geranium thunbergii is a perennial plant commonly used as an oriental medicine for prevention of diarrhea, constipation, and gastrointestinal disorders. However, its anti-inflammatory effect has not been evaluated thus far. Therefore, the present study aimed to investigate the anti-inflammatory effect of G. thunbergii. In this study, G. thunbergii extracted with methanol; this methanol extract was further partitioned using various solvents, and G. thunbergii ethyl acetate fraction (GTEF) was obtained. To determine the anti-inflammatory activity of G. thunbergii, the effects of GTEF on nitric oxide (NO) production in lipopolysaccharide-stimulated RAW 264.7 cells were evaluated. GTEF suppressed NO production in a dose-dependent manner without any toxic effects. In addition, western blotting was performed to examine the effect of GTEF on expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggest that GTEF as a phytoextract may be useful for the prevention or treatment of inflammation.

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Background: Graviola (Annona muricata) is known to have a superior cancer cell killing effect than adriamycin, a chemical drug, and is used in hospitals. Check to see if it actually has an anti-inflammatory effect on the skin.Objective: This study was conducted to assess the anti-inflammatory and molecular effects of A. muricata leaf extract to evaluate its potency as a cosmetic ingredient and value in the cosmetic industry. Methods:The anti-inflammatory effects of A. muricata were evaluated using an ethanolic extract of its dried leaves. To assess the inhibitory effects of A. muricata extract on nitric oxide (NO) production, the amount of NO produced in RAW 264.7 cells induced by lipopolysaccharides (LPS), an inflammatory mediator, was measured. Results:The experiment using the A. muricata leaf extract did not show a significant level of cytotoxicity at all concentration of the extract, and the survival rate of RAW 264.7 cells was low only at high concentrations of the extract. Assessment of the antiinflammatory effects of A. muricata leaf extract showed that a significant amount of NO production was inhibited in the cells compared to that when the leaf extract was incubated with LPS. Conclusion:A. muricata leaf extract would be an excellent candidate as a cosmetic ingredient with its anti-inflammatory properties and other functions.