A high performance liquid chromatography (HPLC) method was developed for the determination of bisphenol A (BPA) that had migrated into canned fruit and vegetables. BPA was extracted with acetonitrile from the solid portion of canned food, and with an OASIS HLB cartridge from the aqueous portion, respectively. Both extracts were cleaned up on a Florisil cartridge. The HPLC separation was carried out on a Wakosil II 3C18 RS column (4.6 x 150 mm) with acetonitrile-water (40:60, v/v) as a mobile phase with a flow rate of 0.8 ml/min. BPA was detectable by UV detector at 228 nm and determined with the similarity of chromatographic peak spectrum by multiwavelength detector (similarity index was 0.99 or above). The quantification limits were 10 ng/g for the solid portion and 5 ng/ml for the aqueous portion, respectively. BPA was mainly detected in the solid portion of canned food and found at the maximum level of 11 micrograms per can. To verify migration into the solid portion of canned food, a partitioning experiment was carried out.

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A simple and rapid method has been developed for the determination of allicin in garlic and commercial garlic products by gas chromatography (GC) with flame photometric detection. Samples containing allicin are homogenized with distilled water and centrifuged. An aliquot of the superaate is cleaned up on an Extrelut column using diethyl ether as an eluant. The GC separation is carried out on a 1% OV-1 or 2% Advance DS+ H3P 0 4 column. The calibration curve was rectilinear in the range from 0.2 to 2.0 pg/mL. The overall recovery of allicin added to garlic and garlic products at 0.4 mg/g was more than 84% with a detection limit of 0.02 mg/g. Allicin was found in spices; whereas, no allicin was found in garlic products marketed as health foods.

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