Abstract:We demonstrate the formation of micropatterned sol-gel structures containing active proteins by patterning with polydimethylsiloxane (PDMS) microchannels. To transport sol solution efficiently into the hydrophobic PDMS microchannels, a hydrophilic-hydrophobic block copolymer was used to impart hydrophilicity to the PDMS microchannels. Poor adhesion of the micropatterned gel structure onto glass slides was improved by treating the glass surface with a polymeric substrate. To minimize cracks in the gel microstructure, hybrid matrices of interpenetrating organic and inorganic networks were prepared containing the reactive organic moieties polyvinylalcohol or polyvinylpyrrolidone. Retention of biochemical activity within the micropatterned gel was demonstrated by performing immunobinding assays with immobilized immunoglobulin G (IgG) antibody. The potential application of microfluidics technology to immobilized-enzyme biocatalysis was demonstrated using PDMS-patterned microchannels filled with trypsin-containing sol-gels. This work provides a foundation for the microfabrication of functional protein chips using sol-gel processes.

In order to test the existence of the sterile neutrino, NEOS experiment was performed from August 2015 to May 2016. The prompt energy spectrum from the inverse beta decay of electron antineutrino from a 2.8 GW th reactor was measured at 24 m distance. The number of measured IBD candidates is about 2000 per day during reactor-on period and a ratio of signal to background is about 22. The result shows no strong evidence of active-to-sterile neutrino. An exclusion-limit curve for 3+1 hypothesis is found at 90% CL. via shape-only analysis.

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