A major modification to the QuEChERS (quick, easy, cheap, effective, rugged and safe) method was developed for the analysis of etoxazole in red pepper using gas chromatography coupled with a nitrogen-phosphorus detector. Etoxazole was extracted with acetonitrile, partitioned with magnesium sulfate and purified with a solid-phase extraction cartridge. The method showed good linearity with a determination coefficient (R(2) ) of 0.998 for the 0.02-2.0 mg/L concentration range. The method was validated using blank red pepper spiked at 0.2 and 1.0 mg/kg, and the average recovery rate was 74.4-79.1% with relative standard deviations <5% for intra- and inter-day precision. The limits of detection and quantification were 0.007 and 0.02 mg/kg, respectively. The developed method was successfully applied to field-incurred samples, and the presence of etoxazole residues was confirmed using gas chromatography/mass spectrometry.
원광대학교 의과대학 산본병원 내과, 2 원광보건대학교 간호학과This study examined the neurotoxicity induced by lead acetate (LA) on cultured C6 glioma cells and the protective effects of Euphorbiae humifusae L. (EH) extract against LA-induced cytotoxicity. In this study, LA exhibited neurotoxicity in a dose-dependent manner compared to the control, and was determined to be highly-toxic according to the toxic criteria. The XTT50 value of LA was calculated at a concentration of 38.6 M after C6 glioma cells were incubated for 72 hours in the media containing 30∼50 M of LA, respectively. In addition, LA-induced neurotoxicity was suggested to correlate with the level of oxidative stress because vitamin E, an antioxidant, increased the cell viability damaged by LA-induced cytotoxicity. The EH extract effectively prevented cell injury from LA-induced cytotoxicity via its antioxidative effects, such as inhibitory ability of lipid peroxidation, superoxide dismutase-like activity and 1,1-diphenyl-2-picrylhydrazyl-radical scavenging activity. These antioxidative effects may result in components, such as polyphenol or flavonoids including gallic acid or quercetin. In conclusion, natural resources, such as EH extracts, may be a useful putative agent for the treatment of diseases associated with oxidative stress, such as lead neurotoxicity.
This study was aimed to evaluate the effect of blending oils on atopic dermatitis (AD) model of NC/Nga mice. Methods: Adult NC/Nga mice were assigned to 1 of 3 groups: control group (C, n= 8), experimental group [AD-induced group, blending oiltreated group (E= 8, 8)]. Mice in the E group were given a treatment of blending oils such as Lavender, Rosemary and Lemon Balm (LRL) for 2 sessions (every day) for 6 days. Results: The AD-induced mice with LRL blending oils treatment showed a significant decrease in epidermal thickness, number of mast cells and degranulation, expression of TNF-α and scoring of sensual assessment. Conclusion: LRL blending oils may be a putative resource for the cure of or treatment of AD by the diminution of AD-pathological factors such as the epidermal thickness, the number of mast cells and degranulation as well as the expression of TNF-α.
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